Recombinant human IL-2 (interleukin 2) stimulates growth and differentiation of cells of the lymphoid lineage, such as T, NK, and B cells. IL-2 is a potent immunomodulatory cytokine, as it prevents autoimmunity and has key functions during infections. IL-2 IS stands for interleukin 2 "Improved Sequence", also termed aldesleukin, and is a variant of IL-2 with a serine substitution for the native cysteine at amino acid position 125. Human IL-2 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays, and possesses the same biological properties as naturally occurring

Data and images for Human IL-2 IS

Figures

Figure 1

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SDS-PAGE of Human IL-2 IS, premium grade
under non-reducing (NR) and reducing (R) conditions.

Figure 1

SDS-PAGE of Human IL-2 IS, premium grade
under non-reducing (NR) and reducing (R) conditions.

Figure 2

View details
Human IL-2 IS biological activity.
Activity of Human IL-2 IS, premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 2

Human IL-2 IS biological activity.
Activity of Human IL-2 IS, premium grade, (red bar) was compared to another commercially available product (black bar).

Specifications for Human IL-2 IS

Overview

Recombinant human IL-2 (interleukin 2) stimulates growth and differentiation of cells of the lymphoid lineage, such as T, NK, and B cells. IL-2 is a potent immunomodulatory cytokine, as it prevents autoimmunity and has key functions during infections. IL-2 IS stands for interleukin 2 "Improved Sequence", also termed aldesleukin, and is a variant of IL-2 with a serine substitution for the native cysteine at amino acid position 125. Human IL-2 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays, and possesses the same biological properties as naturally occurring IL-2.

Applications

Human IL-2 IS can be used for a variety of applications including:
  • In vitro activation and propagation of T cells, e.g., in combination with the T Cell Activation/Expansion Kit, human.
  • In vitro stimulation of cytolytic function and expansion of NK cells, e.g., using the NK Cell Activation/Expansion Kit, human.
  • Generation of lymphokine-activated killer (LAK) cells or cytokine-induced killer (CIK) cells.

Detailed product information

Background information

IL-2, a potent lymphoid cell growth factor, is a typical four α-helix bundle cytokine. It is produced by activated T cells, especially the CD4
+
T helper cell population. It plays an important role in both the activation and maintenance of immune responses and in lymphocyte development. IL-2 promotes proliferation and differentiation of T cells, NK cells and B cells and is involved in the elimination of self-reactive T cells. IL-2 signals through a receptor complex consisting of IL-2 receptor α-chain (CD25), β- chain, and common γ-chain. The latter two are also used for IL-15 signaling.

Biological activity

  • Proliferation of CTLL-2 cells (NIBSC 86/504)
  • research grade: ≥ 3×
    10
    6
    IU/mg
  • premium grade: ≥ 5×
    10
    6
    IU/mg
    (Typical specific activity: ≥ 9×
    10
    6
    IU/mg
    )
  • We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity, exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human IL-2 IS

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human IL-2 IS

Publications

  1. Müller-Durovic, B. et al. (2016) Killer Cell Lectin-like Receptor G1 Inhibits NK Cell Function through Activation of Adenosine 5'-Monophosphate-Activated Protein Kinase. J. Immunol. 197(7): 2891-2899
  2. Thangamani, S. et al. (2015) Cutting edge: progesterone directly upregulates vitamin d receptor gene expression for efficient regulation of T cells by calcitriol. J. Immunol. 194(3): 883-886
  3. Lissandrello, C. A. et al. (2020) High-throughput continuous-flow microfluidic electroporation of mRNA into primary human T cells for applications in cellular therapy manufacturing. Sci Rep 10(1): 180445
  4. Jamali, A. et al. (2020) Highly Efficient Generation of Transgenically Augmented CAR NK Cells Overexpressing CXCR4. Front Immunol 11: 2028
  5. Schipp, C. et al. (2018) EBV Negative Lymphoma and Autoimmune Lymphoproliferative Syndrome Like Phenotype Extend the Clinical Spectrum of Primary Immunodeficiency Caused by STK4 Deficiency. Front Immunol 9: 2400
  6. Zanoni, M. et al. (2020) Innate, non-cytolytic CD8+ T cell-mediated suppression of HIV replication by MHC-independent inhibition of virus transcription. PLoS Pathog. 16(9): e1008821
  7. Lee, H. J. et al. (2017) Expansion of tumor-infiltrating lymphocytes and their potential for application as adoptive cell transfer therapy in human breast cancer. Oncotarget. 8(69): 113345-113359
  8. Niu, C. et al. (2017) Low-dose bortezomib increases the expression of NKG2D and DNAM-1 ligands and enhances induced NK and γδ T cell-mediated lysis in multiple myeloma. Oncotarget. 8(4): 5954-5964
  9. Agarwal, S. et al. (2019) In vivo generated human CAR T cells eradicate tumor cells. Oncoimmunology 8(12): e1671761
  10. Woods, K. et al. (2014) Effects of epithelial to mesenchymal transition on T cell targeting of melanoma cells. Front Oncol. 4: 367
  11. Kamei, R. et al. (2018) Expression levels of UL16 binding protein 1 and natural killer group 2 member D affect overall survival in patients with gastric cancer following gastrectomy. Oncol Lett. 15(1): 747-754
  12. Surenaud, M. et al. (2016) Optimization and evaluation of Luminex performance with supernatants of antigen-stimulated peripheral blood mononuclear cells. BMC Immunol. 17(1): 44
  13. Colson, P. et al. (2014) HIV infection en route to endogenization: two cases. Clin Microbiol Infect 20(12): 1280-1288
  14. Pallett, L. J. et al. (2015) Metabolic regulation of hepatitis B immunopathology by myeloid-derived suppressor cells. Nat Med 21(6): 591-600
  15. Fuchs, S. et al. (2014)
    Patients with T
    +/low
    NK
    +
    IL-2 receptor γ chain deficiency have differentially-impaired cytokine signaling resulting in severe combined immunodeficiency.
    Eur. J. Immunol. 44(10): 3129-3140
  16. Engelmann, S. et al. (2013) T cell-independent modulation of experimental autoimmune encephalomyelitis in ADAP-deficient mice. J. Immunol. 191(10): 4950-4959
  17. Valignat, M. P. et al. (2013) T lymphocytes orient against the direction of fluid flow during LFA-1-mediated migration. Biophys. J. 104(2): 322-331
  18. Fallarini, S. et al. (2012) Invariant NKT cells increase drug-induced osteosarcoma cell death. Br J Pharmakon 167(7): 1533-1549
  19. Ng, S. B. et al. (2011) Dysregulated microRNAs affect pathways and targets of biologic relevance in nasal-type natural killer/T-cell lymphoma. Blood 118(18): 4919-4929
  20. Nguyen, T. L. et al. (2011) Antigen-specific TGF-β-induced regulatory T cells secrete chemokines, regulate T cell trafficking, and suppress ongoing autoimmunity. J. Immunol. 187(4): 1745-1753

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