Alternative names:
TRAP, CD154, TNFSF5

Data and images for Human CD40-Ligand

Figures

Figure 1

View details
Human CD40-Ligand activity assay:
The specific activity is determined by
activation assay
using enriched CD19
+
B cells in the presence of the cross-linking antibody and of 50 IU/mL Interleukin 4. Activity of Human CD40-Ligand, premium grade (red line) was compared to another commercially available product (black line).

Figure 1

Human CD40-Ligand activity assay:
The specific activity is determined by
activation assay
using enriched CD19
+
B cells in the presence of the cross-linking antibody and of 50 IU/mL Interleukin 4. Activity of Human CD40-Ligand, premium grade (red line) was compared to another commercially available product (black line).

Figure 2

View details
SDS-PAGE of Human CD40-ligand, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 2

SDS-PAGE of Human CD40-ligand, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 3

View details
Human CD40-Ligand biological activity.
Activity of Human CD40-Ligand, premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 3

Human CD40-Ligand biological activity.
Activity of Human CD40-Ligand, premium grade, (red bar) was compared to another commercially available product (black bar).

Specifications for Human CD40-Ligand

Overview

Human CD40-Ligand is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human CD40-Ligand can be used for a variety of applications, including:
  • Stimulation of B cell activation and proliferation.
  • Stimulation of dendritic cell maturation.
  • Induction of cytokine production in peripheral blood monocytes and T cells.

Alternative names

TRAP, CD154, TNFSF5

Detailed product information

Background information

CD40-Ligand, also known as CD40L, CD154, TRAP, or TNFSF5 is a member of the TNF superfamily transiently expressed on the surface of activated CD4
+
T lymphocytes. It is either expressed membrane-bound or proteolytically released as a soluble form which comprises 2/3 of the extracellular domain. Its receptor CD40 is found on antigen-presenting cells such as B cells, dendritic cells, and macrophages. CD40L-CD40 interaction is important in T cell-APC (antigen-presenting cell) interaction and is e.g. involved in B cell differentiation and proliferation, isotype class-switching, and protection of B cells from apoptosis.

Biological activity

  • Proliferation of CD19
    +
    B cells
  • premium grade: ≥ 4×
    10
    3
    U/mg
  • We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity, exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www.miltenyibiotec.com/certificates).

Resources for Human CD40-Ligand

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human CD40-Ligand

Publications

  1. Spriggs, M. K. et al. (1992) Recombinant human CD40 ligand stimulates B cell proliferation and immunoglobulin E secretion. J. Exp. Med. 176: 1543-1550
  2. Kuen, J. et al. (2017) Pancreatic cancer cell/fibroblast co-culture induces M2 like macrophages that influence therapeutic response in a 3D model. PLoS One 12(7): e0182039
  3. Monaghan, T. M. et al. (2013) Circulating antibody and memory B-Cell responses to C. difficile toxins A and B in patients with C. difficile-associated diarrhoea, inflammatory bowel disease and cystic fibrosis PLoS One 8(9): e74452
  4. Johnson, M. J. et al. (2018) Transcriptional profiles of human islet and exocrine endothelial cells in subjects with or without impaired glucose metabolism. Sci Rep 8(1): 12144
  5. Koliha, N. et al. (2016) Melanoma affects the composition of blood cell-derived extracellular vesicles. Front Immunol 7: 282
  6. Horns, F. et al. (2016) Lineage tracing of human B cells reveals the in vivo landscape of human antibody class switching. Elife 5: e16578
  7. De Keersmaecker, B. et al. (2020) TriMix and tumor antigen mRNA electroporated dendritic cell vaccination plus ipilimumab: link between T-cell activation and clinical responses in advanced melanoma. J Immunother Cancer 8(1): e000329
  8. Hou, G. et al. (2021) SLE non-coding genetic risk variant determines the epigenetic dysfunction of an immune cell specific enhancer that controls disease-critical microRNA expression. Nat Commun. 12(1): 135

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