Feeder Removal MicroBeads have been developed for the efficient depletion of mouse feeder cells from co-cultures with human or mouse ES and iPS cell lines and can also be used with other, feeder-dependent cell lines. Depletion of feeder cells, e.g. prior to differentiation or gene expression profiling studies, helps to obtain reproducible results.

Data and images for Feeder Removal MicroBeads, mouse

Figures

Figure 1

Mouse embryonic fibroblasts (mEF) were depleted from a co-culture with mouse embryonic stem (mES) cells using Feeder Removal MicroBeads, an LS Column, and a MidiMACS™ Separator. Flow cytometric analysis with CD15 (SSEA-1)-APC and Anti-Feeder-PE on the MACSQuant® Analyzer indicates that a starting population of 10% mEF was completely removed. The purity of the enriched mES cells was about 99.9%.
Unseparated fraction
After separation
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Figure 1

Mouse embryonic fibroblasts (mEF) were depleted from a co-culture with mouse embryonic stem (mES) cells using Feeder Removal MicroBeads, an LS Column, and a MidiMACS™ Separator. Flow cytometric analysis with CD15 (SSEA-1)-APC and Anti-Feeder-PE on the MACSQuant® Analyzer indicates that a starting population of 10% mEF was completely removed. The purity of the enriched mES cells was about 99.9%.
View details

Figure 1

Mouse embryonic fibroblasts (mEF) were depleted from a co-culture with mouse embryonic stem (mES) cells using Feeder Removal MicroBeads, an LS Column, and a MidiMACS™ Separator. Flow cytometric analysis with CD15 (SSEA-1)-APC and Anti-Feeder-PE on the MACSQuant® Analyzer indicates that a starting population of 10% mEF was completely removed. The purity of the enriched mES cells was about 99.9%.

Specifications for Feeder Removal MicroBeads, mouse

Overview

Feeder Removal MicroBeads have been developed for the efficient depletion of mouse feeder cells from co-cultures with human or mouse ES and iPS cell lines and can also be used with other, feeder-dependent cell lines. Depletion of feeder cells, e.g. prior to differentiation or gene expression profiling studies, helps to obtain reproducible results.

Detailed product information

Background information

The most prevalent cultivation method for maintenance of mouse and human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells is coculturing on mouse embryonic fibroblast feeder cell layers. Different feeder cell types are employed, among these primary embryonic fibroblasts (mEF) and mEF-derived cell lines (e.g. NIH3T3). Feeder cells are also used in maintenance cultures of primary keratinocytes.

Downstream applications

Depletion of feeder cells improves the reproducibility of many downstream application, including
  • Gene expression-profiling
  • Blastocyst injection of ES cells after gene targeting
  • Differentiation protocols for ES and iPS cells: Removal of feeder cells can improve the responsiveness to developmental cues.

Columns

LS or autoMACS
®
Columns.

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Feeder Removal MicroBeads, mouse

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