Clone:
REA688
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
EGFR

Extended validation for EGF Receptor Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of EGF Receptor knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with EGF Receptor-PE (REA688, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of EGF Receptor knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with EGF Receptor-PE (REA688, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of EGF Receptor knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with EGF Receptor-PE (REA688, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of EFGR knockout cells. Wild type (red) and knockout cells (blue) were stained with EGFR-PE, clone (REA688). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of EFGR knockout cells. Wild type (red) and knockout cells (blue) were stained with EGFR-PE, clone (REA688). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for EGF Receptor Antibody, anti-human, REAfinity™

Overview

Clone RE688 recognizes the human epidermal growth factor receptor (EGFR) antigen. EGFR is a single-pass type I membrane protein which is also known as receptor tyrosine-protein kinase ErbB-1 or HER1. It is a critical regulator of many normal cellular processes, including cell growth, differentiation, survival, and migration. The EGFR also is implicated in pathological processes of cellular transformation and oncogenesis due to its overexpression in many types of cancers and its ability to induce morphological transformation of cultured cells and tumor formation in nude mice. Many different signaling pathways have been discovered to mediate the effects of EGF, the most notable being Ras-mitogen-activated protein kinase (MAPK) and the phosphatidylinositol 3-kinase pathways. In these pathways EGF binds to the EGFR to induce dimerization, catalytic activation, and autophosphorylation of tyrosines in the C-terminal tail of the EGFR. These phosphorylated tyrosines provide docking sites for adapter proteins such as Grb2, Shc, and Gab2 to link the receptor to the Ras-MAPK, as well as phosphatidylinositol 3-kinase pathways.
Additional information: Clone REA688 displays negligible binding to Fc receptors.

Alternative names

EGFR

Detailed product information

Technical specifications

CloneREA688
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenEGF Receptor
Alternative names of antigenEGFR
Molecular mass of antigen [kDa]132
Distribution of antigenother, kidney, prostate
Entrez Gene ID1956
RRIDAB_2651596, AB_2651597, AB_2651598, AB_2651599, AB_2651600, AB_2651601, AB_2651602, AB_2651603, AB_2651595

Resources for EGF Receptor Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for EGF Receptor Antibody, anti-human, REAfinity™

Publications

  1. Ullrich, A. et al. (1984) Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells. Nature 309(5967): 418-425
  2. Bell, G. I. et al. (1986)
    Human epidermal growth factor precursor: cDNA sequence, expression
    in vitro
    and gene organization.
    Nucleic Acids Res. 14(21): 8427-8446
  3. Heisermann, G. J. et al. (1988)
    Epidermal growth factor receptor threonine and serine residues phosphorylated
    in vivo
    .
    J. Biol. Chem. 263(26): 13152-13158

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