Cytokeratin Antibody, anti-human, REAfinity™

Name: Cytokeratin Antibody, anti-human, REAfinity™
Availability: InStock

Cytokeratin Antibody, anti-human, REAfinity™Extended ValidationRecombinant

Clone:

REA801

Type of antibody:

Primary antibodies, Recombinant antibodies

Isotype:

recombinant human IgG1

Applications:

ICFC

Alternative names:

KRT8, KRT18, KRT19

Intracellular flow cytometry applications forCytokeratin Antibody, anti-human, REAfinity™

APC
PE
pure

Conjugate:

APC

Recommended dilution:

1:50

Remark:

The antibody is suited for staining of formaldehyde-fixed cells.

Tested:

QC tested

Products used:

130-112-347, 130-112-190

Protocols:

Intracellular flow cytometry staining protocol (Inside Stain Kit 1:50)

Description:

SK-BR-3 cells were fixed and permeabilized followed by intracellular staining with Anti-Cytokeratin antibodies or with the corresponding REA Control (I) antibodies (left peak). Flow cytometry was performed using the MACSQuant^® Analyzer. Cell debris were excluded from the analysis based on scatter signals.

Extended validation for Cytokeratin Antibody, anti-human, REAfinity™

Specificity

Epitope competition

In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.

Other clones	Overlap in epitope recognition with REA801
CAM5.2	-
JJB3	-
C-11	-
Cells were incubated with an excess of purified unconjugated Cytokeratin (REA801) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for Cytokeratin. Human SK-BR-3 cells stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with Cytokeratin antibodies and plotted against the side scatter. As a control, Cytokeratin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Cytokeratin. Human SK-BR-3 cells stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with Cytokeratin antibodies and plotted against the side scatter. As a control, Cytokeratin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Cytokeratin. Human SK-BR-3 cells stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with Cytokeratin antibodies and plotted against the side scatter. As a control, Cytokeratin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for Cytokeratin Antibody, anti-human, REAfinity™

Overview

Clone REA801 specifically recognizes cytokeratin 8, 18, and 19. It almost completely crossblocks A45-B/B3, an antibody known to recognize the cytokeratin heterodimers 8/18 and 8/19. Cytokeratins are located in the intracytoplasmic cytoskeleton of epithelial tissue.

Additional information: Clone REA801 displays negligible binding to Fc receptors.

Alternative names

KRT8, KRT18, KRT19

Detailed product information

Technical specifications

Clone	REA801
Clonality	monoclonal
Isotype	recombinant human IgG1
Isotype control	REA Control Antibody (I), human IgG1
Host	human cell line
Type of antibody	Primary antibodies, Recombinant antibodies
Species	human
Antigen	Cytokeratin
Alternative names of antigen	KRT8, KRT18, KRT19
Molecular mass of antigen [kDa]	44-53
Distribution of antigen	epithelial cells
Entrez Gene ID	3856, 3875, 3880
RRID	AB_2651491, AB_2651492, AB_2651493, AB_2651494, AB_2651490

Resources for Cytokeratin Antibody, anti-human, REAfinity™

Certificates

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References for Cytokeratin Antibody, anti-human, REAfinity™

Publications

Oshima, R. G. et al. (1986) Comparison of mouse and human keratin 18: a component of intermediate filaments expressed prior to implantation. Differentiation 33(1): 61-68
Waseem, A. et al. (1990) Embryonic simple epithelial keratins 8 and 18: chromosomal location emphasizes difference from other keratin pairs. New Biol. 2(5): 464-478

Applications

Extended validation