CD79a antibodies

CD79a antibodies

Clone: HM47
HM47 recognizes the cytoplamic domain of human CD79a, which is also known as mb-1 and Ig α. Along with CD79b (Ig β), CD79a is associated with surface Ig (sIg) to form the B cell antigen receptor complex. CD79a is a 47 kDa glycoprotein and comprises a single extracellular immunoglobulin domain, a transmembrane domain, and an signaling intracellular domain with immunoreceptor tyrosine-based activation motif (ITAM). CD79a/CD79b heterodimer facilitates differentiation of pre-B cells from pro-B cells, surface expression of sIg, signal transduction following antigen recognition and endocytosis of recognized antigens. Surface expression of CD79a diminishes in plasma cells, where it is mainly found as an intracellular molecule. CD79 is considered a B cell–specific marker and is often used to identify the B cell lineage of acute lymphoblastic leukemia. Recent reports however suggest expression of CD79a in T cell acute lymphoblastic leukemia. Individuals lacking CD79a have low/absent circulating pre-B cells and serum antibodies due to a block in B cell development.

Applications

Reagent can be used for immunophenotyping by flow cytometry. Abnormal numbers of cells expressing this antigen or aberrant expression levels of the antigen can be expected in some disease states. It is important to understand the normal expression pattern for this antigen and its relationship to expression of other relevant antigens in order to perform appropriate analysis.
Expression of CD79a may be used as aid to diagnostic in the characterization of samples from individuals suspected with hematologic neoplasia.

Technical specifications

  • Antigen: CD79a
  • Clone: HM47
  • Isotype: mouse IgG1κ
  • Molecular mass of antigen [kDa]: 22
  • Distribution of antigen: B cells, lymphocytes, plasma cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE
  • Selected References

    1. Lin, J. and Justement, L. B. (1992) The MB-1/B29 heterodimer couples the B cell antigen receptor to multiple src family protein tyrosine kinases. J. Immunol. 149: 1548-1555
    2. Clinical and Laboratory Standards Institute (CLSI) (2007) Clinical Flow Cytometric Analysis of Neoplastic Hematolympoid Cells, CLSI document H43-A2 (ISBN 1-56238-635-2) CLSI; Approved Guideline - Second Edition
    3. Lai, R. et al. (2000) Flow cytometric detection of CD79a expression in T-cell acute lymphoblastic leukemias. Am. J. Clin. Pathol. 113(6): 823-830
    4. van Dongen, J. J. M. et al. (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9): 1908-1975
    5. Rothe, G et al. (2012) Consensus protocol for the flow cytometric Immunophenotyping of hematopoietic malignancies. Leukemia 10(5): 877-895
    6. Stelzer GT et al. (1997) U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 30(5): 214-230
    7. Koyama, M. et al. (1997) CD79α/CD79β heterodimers are expressed on pro-B cell surfaces without associated μ heavy chain. Int. Immunol. 9: 1767-1772
    8. Minegishi, Y. et al. (1999) Mutations in Igalpha (CD79a) result in a complete block in B-cell development. J. Clin. Invest. 104(8): 1115-1121
    9. Mason, D. Y. et al. (1991) The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J. Immunol. 147(11): 2474-2482
    10. Clinical and Laboratory Standards Institute (CLSI) (2007) Enumeration of Immunologically Defined Cell Populations by Flow Cytometry, CLSI document H42-A2 (ISBN 1-56238-640-9) CLSI; Approved Guideline - Second Edition
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Product information

Size
Order no.
Price

CD79a-PE

  • HM47
  • CE–IVD
for 100
tests
170-078-035
EUR 530,00