CD64 antibodies

CD64 antibodies

Clone: 10.1.1
10.1.1 recognizes human CD64, a high affinity Fc receptor for IgG. CD64 contains three Ig-like domains in its extracellular domain and binds both monomeric and aggregated IgG and contributes to antibody-dependent cellular cytotoxicity (ADCC), phagocytosis, and the clearance of immune complexes. It is constitutively expressed on macrophages, monocytes, and eosinophils and can be up-regulated on neutrophils during infection or after stimulation with IFN-γ and colony-stimulating factors G-CSF and GM-CSF.

Applications

Reagent can be used for immunophenotyping by flow cytometry. Abnormal numbers of cells expressing this antigen or aberrant expression levels of the antigen can be expected in some disease states. It is important to understand the normal expression pattern for this antigen and its relationship to expression of other relevant antigens in order to perform appropriate analysis.
Expression of CD64 may be used as aid to diagnostic in the characterization of samples from individuals suspected with hematologic neoplasia.

Technical specifications

  • Antigen: CD64
  • Clone: 10.1.1
  • Isotype: mouse IgG1κ
  • Molecular mass of antigen [kDa]: 41
  • Distribution of antigen: dendritic cells, eosinophils, macrophages, monocytes, myeloid cells, neutrophils
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE
  • Selected References

    1. Buckle, A. M. and Hogg, N. (1989) The effect of IFN-gamma and colony-stimulating factors on the expression of neutrophil cell membrane receptors. J. Immunol. 143(7): 2295-2301
    2. Clinical and Laboratory Standards Institute (CLSI) (2007) Enumeration of Immunologically Defined Cell Populations by Flow Cytometry, CLSI document H42-A2 (ISBN 1-56238-640-9) CLSI; Approved Guideline - Second Edition
    3. Clinical and Laboratory Standards Institute (CLSI) (2007) Clinical Flow Cytometric Analysis of Neoplastic Hematolympoid Cells, CLSI document H43-A2 (ISBN 1-56238-635-2) CLSI; Approved Guideline - Second Edition
    4. Schlossman, S.F. et al. (1993) Leukocyte Typing V. Oxford, Oxford University Press
    5. McMichael, A.J. et al. (1987) Leucocyte typing III. White cell differentiation Antigens. Oxford, Oxford University Press : 91
    6. van Dongen, J. J. M. et al. (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9): 1908-1975
    7. Rothe, G et al. (2012) Consensus protocol for the flow cytometric Immunophenotyping of hematopoietic malignancies. Leukemia 10(5): 877-895
    8. Stelzer GT et al. (1997) U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 30(5): 214-230
    9. Wood BL et al. (2007) 2006 Bethesda International Consensus recommendations on the immunophenotypic analysis of hematolymphoid neoplasia by flow cytometry: optimal reagents and reporting for the flow cytometric diagnosis of hematopoietic neoplasia. Cytometry B Clin. Cytom. 72: 14-22
    10. Herra, C. et al. (1996) Increased expression of Fc gamma receptors on neutrophils and monocytes may reflect ongoing bacterial infection. J. Med. Microbiol. 44(2): 135-140
    11. Shen, L. et al. (1987) Polymorphonuclear leukocyte function triggered through the high affinity Fc receptor for monomeric IgG. J. Immunol. 139(2): 534-538
    12. Kishimoto, T et al. (eds) Leucocyte typing VI: White cell differentiation antigens. Garland Publishing Inc., New York
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  • Certificates of Analysis (CoA)

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Product information

Size
Order no.
Price

CD64-PE

  • 10.1.1
  • CE–IVD
for 100
tests
170-078-085
EUR 420,00