Clone:
REA835
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC, IF, IHC, MICS, 3D-IF
Alternative names:
Lamp4, SCARD1, gp110, macrosialin

Extended validation for CD68 Antibody, anti-mouse, REAfinity™

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD68. Mouse splenocytes were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with CD68 antibodies. As a control, CD68 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/452 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD68. Mouse splenocytes were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with CD68 antibodies. As a control, CD68 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/452 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD68. Mouse splenocytes were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with CD68 antibodies. As a control, CD68 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/452 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD68. Mouse splenocytes were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with CD68 antibodies. As a control, CD68 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/452 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD68. Mouse splenocytes were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with CD68 antibodies. As a control, CD68 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/452 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD68. Mouse splenocytes were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with CD68 antibodies. As a control, CD68 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/452 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for CD68 Antibody, anti-mouse, REAfinity™

Overview

Clone REA835 recognizes mouse CD68 antigen, also known as macrosialin, which is a heavily glycosylated type I transmembrane protein of the lysosomal-associated membrane protein (LAMP) family. It is expressed on tissue macrophages, Langerhans cells, and at a low density on dendritic cells. Murine CD68 is predominantly intracellular but it is reported that 10–15% of CD68 is expressed on the cell surface as well. Macrosialin functions as a macrophage receptor for oxidized low density lipoprotein.
Additional information: Clone REA835 displays negligible binding to Fc receptors.

Alternative names

Lamp4, SCARD1, gp110, macrosialin

Detailed product information

Technical specifications

CloneREA835
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD68
Alternative names of antigenLamp4, SCARD1, gp110, macrosialin
Molecular mass of antigen [kDa]33
Distribution of antigenmacrophages, dendritic cells, Langerhans cells
Entrez Gene ID12514
RRIDAB_2659022, AB_2659023, AB_2659024, AB_2659025, AB_2659026, AB_2659027, AB_2659028, AB_2659029, AB_2659030, AB_2659031, AB_2659032, AB_2659033, AB_2659034, AB_2659035, AB_2659036, AB_2659037, AB_2659038, AB_2659039, AB_2659040, AB_2905105, AB_2659021

Resources for CD68 Antibody, anti-mouse, REAfinity™

Certificates

Please follow this
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to search for Certificates of Analysis (CoA) by lot number.

References for CD68 Antibody, anti-mouse, REAfinity™

Publications

  1. Smith, M. J. et al. (1987) Differential expression of murine macrophage surface glycoprotein antigens in intracellular membranes. J. Cell. Sci. 87: 113-119
  2. Ramprasad, M. P. et al. (1996) Cell surface expression of mouse macrosialin and human CD68 and their role as macrophage receptors for oxidized low density lipoprotein. Proc. Natl. Acad. Sci. U.S.A. 93: 14833-14838
  3. Kurushima, H. et al. (2000) Surface expression and rapid internalization of macrosialin (mouse CD68) on elicited mouse peritoneal macrophages. J. Leukoc. Biol. 67: 104-108

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