Clone:
TS2/9
Type of antibody:
Primary antibodies, Functional-grade antibodies
Isotype:
mouse IgG1κ
Applications:
FC, FA, MICS, IF, IHC
Alternative names:
LFA-3, ag3

Extended validation for CD58 (LFA-3) Antibody, anti-human

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
View details
Overlay histogram showing flow cytometric analysis of CD58 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD58-PE, clone (TS29). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD58 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD58-PE, clone (TS29). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD58 (LFA-3) Antibody, anti-human

Overview

The monoclonal antibody TS2/9 recognizes human CD58, a 45–70 kDa cell surface molecule which is a member of the immunoglobin superfamily. It is expressed on professional antigen-presenting cells (APCs) such as B cells, dendritic cells, macrophages, and epithelial cells as well as on T cells, monocytes, erythrocytes, endothelial cells, and fibroblasts. LFA-3 interacts with CD2 on T cells and is important for interaction between T cells and professional APCs.

Alternative names

LFA-3, ag3

Detailed product information

Technical specifications

CloneTS2/9
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies, Functional-grade antibodies
Specieshuman
AntigenCD58 (LFA-3)
Alternative names of antigenLFA-3, ag3
Distribution of antigenB cells, dendritic cells, endothelial cells, macrophages, T cells
RRIDAB_2801757, AB_2658757, AB_2658758, AB_2658759, AB_2658760, AB_2658761, AB_2658764, AB_2658765, AB_2801760

Resources for CD58 (LFA-3) Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD58 (LFA-3) Antibody, anti-human

Publications

  1. Sanchez-Madrid, F. et al. (1982) Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. Proc. Natl. Acad. Sci. U.S.A. 79: 7489-7493
  2. Dengler, T. J. et al. (1992) Structural and functional epitopes of the human adhesion receptor CD58 (LFA-3). Eur. J. Immunol. 22(11): 2809-2817
  3. Wang, J. H. et al. (1999) Structure of a heterophilic adhesion complex between the human CD2 and CD58 (LFA-3) counterreceptors. Cell 97(6): 791-803

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