Clone:
23C6
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC, MICS, IF, IHC
Alternative names:
ITGAV, MSK8, VNRA, VTNR, BDPLT16, BDPLT2, CD61, GP3A, GPIIIa, ITGB3

Extended validation for CD51/CD61 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with 23C6
REA1099++
NKI-M9-
P1F6-
REA181++
Cells were incubated with an excess of purified unconjugated CD51/CD61 (23C6) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD51/CD61. Human fibroblast cells were stained with CD51/CD61 antibodies and plotted against the side scatter. As a control, CD51/CD61 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD51/CD61. Human fibroblast cells were stained with CD51/CD61 antibodies and plotted against the side scatter. As a control, CD51/CD61 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD51/CD61. Human fibroblast cells were stained with CD51/CD61 antibodies and plotted against the side scatter. As a control, CD51/CD61 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD51/CD61. Human fibroblast cells were stained with CD51/CD61 antibodies and plotted against the side scatter. As a control, CD51/CD61 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD51/CD61. Human fibroblast cells were stained with CD51/CD61 antibodies and plotted against the side scatter. As a control, CD51/CD61 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD51/CD61 (23C6). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD51/CD61 (23C6). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD51/CD61 (23C6). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD51/CD61 Antibody, anti-human

Overview

The monoclonal antibody 23C6 recognizes the human heterodimeric complex CD51/CD61, also known as integrin αv/β3. The dimers are expressed on melanoma cells, endothelial cells, on osteoclasts, and in a very low density on platelets. The CD51/CD61 complex mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, osteopontin, and collagen.

Alternative names

ITGAV, MSK8, VNRA, VTNR, BDPLT16, BDPLT2, CD61, GP3A, GPIIIa, ITGB3

Detailed product information

Technical specifications

Clone23C6
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, other
Cross-reactivityrabbit, chicken
AntigenCD51/CD61
Alternative names of antigenITGAV, MSK8, VNRA, VTNR, BDPLT16, BDPLT2, CD61, GP3A, GPIIIa, ITGB3
Molecular mass of antigen [kDa]197(sum of molecular weights of subunits)
Distribution of antigenendothelial cells, osteoclasts
Entrez Gene ID3685, 3690
RRIDAB_2658629, AB_2658630, AB_2658631, AB_2658632, AB_2658633, AB_2658634, AB_2658635, AB_2658636, AB_2658637, AB_2658638, AB_2658639, AB_2658640, AB_2658641, AB_2658628

Resources for CD51/CD61 Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD51/CD61 Antibody, anti-human

Publications

  1. Chuntharapai, A. et al. (1993) Blocking monoclonal antibodies to alpha V beta 3 integrin: a unique epitope of alpha V beta 3 integrin is present on human osteoclasts. Exp. Cell Res. 205(2): 345-352
  2. Athanasou, N. A. et al. (1990) New sites of cellular vitronectin receptor immunoreactivity detected with osteoclast-reacting monoclonal antibodies 13C2 and 23C6. Bone Miner. 8(1): 7-22

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