CD45RO Antibody, anti-human,
REAlease
^®

Name: CD45RO Antibody, anti-human, REAlease®
Availability: InStock

CD45RO Antibody, anti-human,
REAlease
^®

Clone:

REAL377

Type of antibody:

Releasable antibodies, Primary antibodies, Recombinant antibodies

Applications:

FC, MICS, IF, IHC

Flow cytometry

Conjugate:	PE
Recommended dilution:	1:50
Tested:	QC tested
Products used:	130-120-961, 130-120-872, 130-124-842

MICS (MACSima Imaging Cyclic Staining)

Conjugate:	PE
Recommended dilution:	1:50
Fixation:	FFPE
Tested:	during development
Products used:	130-120-961, 130-120-872
Compatible applications:	IF, IHC

Extended validation for CD45RO Antibody, anti-human,
REAlease
^®

Specificity

Knockout validation

To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.

WT

KO

View details

WT

Fluorescence microscopy image of CD45RO knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD45RO-PE (REAL377, red) and counterstained with DRAQ5 (blue) as DNA stain.

View details

KO

Fluorescence microscopy image of CD45RO knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD45RO-PE (REAL377, red) and counterstained with DRAQ5 (blue) as DNA stain.

View details

Overlay histogram showing flow cytometric analysis of human CD45RO knockout cells. Wild type (red) and knockout cells (blue) were stained with CD45RO-PE, clone (REAL377). Flow cytometry was performed with the MACSQuant

^®

Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Overlay histogram showing flow cytometric analysis of human CD45RO knockout cells. Wild type (red) and knockout cells (blue) were stained with CD45RO-PE, clone (REAL377). Flow cytometry was performed with the MACSQuant

^®

Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD45RO Antibody, anti-human,
REAlease
^®

Overview

Clone REAL377 is an antibody fragment derived from the full CD45RO antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.

Clone REAL377 recognizes the human CD45RO antigen, a 180 kDa single-chain membrane glycoprotein, which is an isoform of CD45. CD45RO is expressed by most thymocytes, activated and memory T cells, granulocytes, B cell subsets, and monocytes. CD45RO binds to galectin-1 and CD22. CD45RO antibodies are commonly used in combination with antibodies against CD45RA to discriminate memory T cells from naive T cells.

The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Detailed product information

Technical specifications

Clone	REAL377
Clonality	monoclonal
Isotype control	Control Antibody
Host	cell line
Type of antibody	Releasable antibodies, Primary antibodies, Recombinant antibodies
Species	human
Antigen	CD45RO
Distribution of antigen	granulocytes, T cells, B cells, monocytes, thymocytes
RRID	AB_2784223, AB_2784222, AB_2784221, AB_2811684, AB_2811683, AB_2784224

Applications

Extended validation