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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Our local employees are always happy to answer your questions. Highly trained and experienced teams in your country can provide quick, helpful, and comprehensive support.
Miltenyi Biotec B.V.
Sandifortdreef 17
2333 ZZ Leiden
The Netherlands
Phone: 0800 94016
Fax: 0800 99626
E-Mail: macs@miltenyibiotec.nl
Web: www.miltenyibiotec.com
For immediate technical support, use our live chat. Connect with us
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
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Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD45RO Complexes or with the corresponding REAL Control (left images) as well as with CD45RA antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.
Immunofluorescence analysis of a human spleen cryosection fixed with ice-cold acetone. The section was stained using CD45RO Antibody, anti-human, APC (green) for 10 min at room temperature (A). CD45RO Antibody, anti-human, APC staining was released using the REAlease Release Reagent (B). Nuclei were visualized with Hoechst™ (blue). Scale bar: 30 µm.
Immunofluorescence analysis of a formalin-fixed, paraffin embedded (FFPE) human spleen. Before staining, antigens were retrieved using heat-induced epitope retrieval (HIER) at pH 9. The section was stained using CD45RO Antibody, anti-human, APC (green) for 10 min at room temperature (A). CD45RO Antibody, anti-human, APC staining was released using the REAlease Release Reagent (B). Nuclei were visualized with Hoechst™ (blue).Scale bar: 30 µm.
Clone | REAL377 |
---|---|
Clonality | monoclonal |
Isotype control | Control Antibody |
Host | human cell line |
Type of antibody | Releasable antibodies, Primary antibodies, Recombinant antibodies |
Species | human |
Antigen | CD45RO |
Distribution of antigen | granulocytes, T cells, B cells, monocytes, thymocytes |
RRID | AB_2811683, AB_2784223, AB_2784222, AB_2784221, AB_2811684, AB_2784224 |
Clone | REAL377 |
---|---|
Clonality | monoclonal |
Isotype control | Control Antibody |
Host | human cell line |
Type of antibody | Releasable antibodies, Primary antibodies, Recombinant antibodies |
Species | human |
Antigen | CD45RO |
Distribution of antigen | granulocytes, T cells, B cells, monocytes, thymocytes |
RRID | AB_2811683, AB_2784223, AB_2784222, AB_2784221, AB_2811684, AB_2784224 |
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