Clone:
C363-16A
Type of antibody:
Primary antibodies
Isotype:
rat IgG2aκ
Applications:
FC, MICS, IF, IHC
Alternative names:
Ptprc, B220, CD45R, CD45, L-CA, Ly-5, Lyt-4, T200, loc

Extended validation for CD45RB Antibody, anti-mouse

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with C363-16A
16A++
REA1180++
MB4B4++
Cells were incubated with an excess of purified unconjugated CD45RB (C363-16A) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD45RB Antibody, anti-mouse

Overview

The monoclonal antibody C363-16A reacts with an isoform of mouse CD45 known as CD45RB, which is exon B dependent. It is a 220 kDa glycoprotein expressed at a high density on peripheral B cells, on a subpopulation of T cells and thymocytes and is weakly expressed on macrophages and dendritic cells.

Alternative names

Ptprc, B220, CD45R, CD45, L-CA, Ly-5, Lyt-4, T200, loc

Detailed product information

Technical specifications

CloneC363-16A
Clonalitymonoclonal
Isotyperat IgG2aκ
Isotype controlIsotype Control Antibody, rat IgG2a
Hostrat
Type of antibodyPrimary antibodies
Speciesmouse
AntigenCD45RB
Alternative names of antigenPtprc, B220, CD45R, CD45, L-CA, Ly-5, Lyt-4, T200, loc
Molecular mass of antigen [kDa]134(calculated based on gene prediction)
Distribution of antigenB cells, dendritic cells, granulocytes, macrophages, mast cells, monocytes, NK cells, T cells, lymphocytes, basophils, plasma cells, thymocytes
Entrez Gene ID19264
RRIDAB_2658358, AB_2658359, AB_2658360, AB_2658361, AB_2658362, AB_2658363, AB_2658364, AB_2658365

Resources for CD45RB Antibody, anti-mouse

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD45RB Antibody, anti-mouse

Publications

  1. Pulido, R. et al. (1988) Comparative biochemical and tissue distribution study of four distinct CD45 antigen specificities J. Immunol. 140: 3851-3857
  2. Johnson, P. et al. (1989) Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies. J. Exp. Med. 169: 1179
  3. Bottomly, K. et al. (1989) A monoclonal antibody to murine CD45R distinguishes CD4 T cell populations that produce different cytokines. Eur. J. Immunol. 19(4): 617-623
  4. Birkeland, M. L. et al. (1988) Epitopes on CD45R [T200] molecules define differentiation antigens on murine B and T lymphocytes. J. Mol. Cell. Immunol 4(2): 71-85

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