CD43 Antibody, anti-mouse
Clone:
L11
Type of antibody:
Primary antibodies
Isotype:
rat IgG2a
Applications:
FC, MICS, IF, IHC
Alternative names:
Spn, Leukosialin, GALGP, Ly-48, gpL115

Extended validation for CD43 Antibody, anti-mouse

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with L-11
S7++
S11-
1B11-
eBioR2/60n/a
REA840++
Cells were incubated with an excess of purified unconjugated CD43 (L11) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD43. Splenocytes from C57BL/6 were stained with CD43 antibodies and with a suitable counterstaining. As a control, CD43 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD43. Splenocytes from C57BL/6 were stained with CD43 antibodies and with a suitable counterstaining. As a control, CD43 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD43. Splenocytes from C57BL/6 were stained with CD43 antibodies and with a suitable counterstaining. As a control, CD43 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD43. Splenocytes from C57BL/6 were stained with CD43 antibodies and with a suitable counterstaining. As a control, CD43 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD43. Splenocytes from C57BL/6 were stained with CD43 antibodies and with a suitable counterstaining. As a control, CD43 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD43. Splenocytes from C57BL/6 were stained with CD43 antibodies and with a suitable counterstaining. As a control, CD43 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for CD43 Antibody, anti-mouse

Overview

Clone L11 recognizes the mouse CD43 (Ly-48) antigen which is expressed on IL-7–responsive pro–B cells, plasma cells, peritoneal and spleen CD5
+
B cells (B-1 cells), granulocytes, monocytes, macrophages, platelets, NK cells, thymocytes, peripheral cytotoxic T cells, and most T helper cells. CD43 is not expressed on resting, conventional peripheral B cells. Thus, resting B cells are easily identified by the lack of CD43 expression.

Alternative names

Spn, Leukosialin, GALGP, Ly-48, gpL115

Detailed product information

Technical specifications

CloneL11
Clonalitymonoclonal
Isotyperat IgG2a
Isotype controlIsotype Control Antibody, rat IgG2a
Hostrat
Type of antibodyPrimary antibodies
Speciesmouse
AntigenCD43
Alternative names of antigenSpn, Leukosialin, GALGP, Ly-48, gpL115
Molecular mass of antigen [kDa]38
Distribution of antigenB cells, granulocytes, macrophages, megakaryocytes, monocytes, NK cells, platelets, red blood cells, T cells, stem cells, basophils, mesenchymal stem cells, neutrophils, plasma cells, thymocytes, bone marrow
Entrez Gene ID20737
RRIDAB_2661308, AB_2661309, AB_2661310, AB_2661311, AB_2661312, AB_2661307

Resources for CD43 Antibody, anti-mouse

Certificates

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