Clone:
REA760
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
BDPLT10, CHDS7, FAT, GP3B, GP4, GPIV, PASIV, Scarb3, gpIIIb

Extended validation for CD36 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA760
5-271++
CB38++
CLB-IVC7++
NL07++
AC106++
Cells were incubated with an excess of purified unconjugated CD36 (REA760) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD36. Human peripheral blood mononuclear cells (PBMCs) were stained with CD36 antibodies and with a suitable counterstaining. As a control, CD36 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used
View details
Flow cytometric comparison of different clones for CD36. Human peripheral blood mononuclear cells (PBMCs) were stained with CD36 antibodies and with a suitable counterstaining. As a control, CD36 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used
View details
Flow cytometric comparison of different clones for CD36. Human peripheral blood mononuclear cells (PBMCs) were stained with CD36 antibodies and with a suitable counterstaining. As a control, CD36 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used
View details
Flow cytometric comparison of different clones for CD36. Human peripheral blood mononuclear cells (PBMCs) were stained with CD36 antibodies and with a suitable counterstaining. As a control, CD36 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used
View details
Flow cytometric comparison of different clones for CD36. Human peripheral blood mononuclear cells (PBMCs) were stained with CD36 antibodies and with a suitable counterstaining. As a control, CD36 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used
View details
Flow cytometric comparison of different clones for CD36. Human peripheral blood mononuclear cells (PBMCs) were stained with CD36 antibodies and with a suitable counterstaining. As a control, CD36 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used
View details
Flow cytometric comparison of different clones for CD36. Human peripheral blood mononuclear cells (PBMCs) were stained with CD36 antibodies and with a suitable counterstaining. As a control, CD36 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used
Flow cytometric comparison of different clones for CD36. Human peripheral blood mononuclear cells (PBMCs) were stained with CD36 antibodies and with a suitable counterstaining. As a control, CD36 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD36 (REA760). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD36 (REA760). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD36 (REA760). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD36 Antibody, anti-human, REAfinity™

Overview

Clone REA760 recognizes the human CD36 antigen, an integral membrane protein, which is a member of the class B scavenger receptor family of cell surface proteins. CD36 is expressed on various epithelial and endothelial cells as well as erythrocytes, platelets, monocytes, and macrophages, and some macrophage-derived dendritic cells. CD36 functions as a scavenger receptor for long chain fatty acids, oxidized LDL, collagen type I, IV, and V, and thrombospondin, as well as for apoptotic cells. CD36 is an early marker of erythroid differentiation.
Additional information: Clone REA760 displays negligible binding to Fc receptors.

Alternative names

BDPLT10, CHDS7, FAT, GP3B, GP4, GPIV, PASIV, Scarb3, gpIIIb

Detailed product information

Technical specifications

CloneREA760
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD36
Alternative names of antigenBDPLT10, CHDS7, FAT, GP3B, GP4, GPIV, PASIV, Scarb3, gpIIIb
Molecular mass of antigen [kDa]53
Distribution of antigenB cells, dendritic cells, endothelial cells, macrophages, monocytes, platelets, red blood cells
Entrez Gene ID948
RRIDAB_2657727, AB_2657728, AB_2657729, AB_2657730, AB_2657731, AB_2657732, AB_2657733, AB_2657734, AB_2657735, AB_2657736, AB_2657737, AB_2657738, AB_2657739, AB_2657740, AB_2657741, AB_2657742, AB_2657743, AB_2819615, AB_2657726

Resources for CD36 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD36 Antibody, anti-human, REAfinity™

Publications

  1. Tandon, N. N. et al. (1989) Isolation and characterization of platelet glycoprotein IV (CD36). J. Biol. Chem. 264(13): 7570-7575
  2. Armesilla, A. L. et al. (1994) Structural organization of the gene for human CD36 glycoprotein. J. Biol. Chem. 269(29): 18985-18991
  3. Silverstein, R. L. et al. (2009) CD36, a scavenger receptor involved in immunity, metabolism, angiogenesis, and behavior. Sci Signal 2(72)

Related products for
CD36 Antibody, anti-human, REAfinity™

3 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?