CD352 (NTB-A) Antibody, anti-mouse

Name: CD352 (NTB-A) Antibody, anti-mouse
Availability: InStock

CD352 (NTB-A) Antibody, anti-mouseExtended Validation

Clone:

13G3

Type of antibody:

Primary antibodies

Isotype:

mouse IgG2aκ

Applications:

FC, MICS, IF, IHC

Alternative names:

SLAM family receptor, SLAMF6, SLAM F6, LY108, Lymphocyte antigen 108, SLAM family member 6

Flow cytometry applications forCD352 (NTB-A) Antibody, anti-mouse

APC
APC-Vio 770
Biotin
FITC
PE
PE-Vio 770

Conjugate:

APC

Recommended dilution:

1:10

Remark:

Cells should be stained prior to fixation, if formaldehyde is used as a fixative.

Tested:

QC tested

Products used:

130-109-903, 130-109-861

Protocols:

Cell surface flow cytometry staining protocol (PBS/EDTA/BSA 1:10)

Description:

Splenocytes from C57BL/6 mice were stained with CD352 (NTB-A) antibodies or with the corresponding isotype control antibodies (left image) as well as with CD45R (B220) antibodies. Flow cytometry was performed using the MACSQuant^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

MICS (MACSima Imaging Cyclic Staining) applications forCD352 (NTB-A) Antibody, anti-mouse

APC
FITC
PE

Conjugate:

APC

Recommended dilution:

1:10

Fixation:

Acetone

Species tested:

mouse

Products used:

130-109-903, 130-109-861

Compatible applications:

IF, IHC

Protocols:

Protocol for MICS experiments

Extended validation for CD352 (NTB-A) Antibody, anti-mouse

Specificity

Epitope competition

In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.

Other clones	Overlap in epitope recognition with 13G3
REA1097	++
330-AJ	+
13G3-19D	++
Cells were incubated with an excess of purified unconjugated CD352 (NTB-A) (13G3) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for CD352 (NTB-A). Splenocytes from C57BL/6 were stained with CD352 (NTB-A) antibodies and with a suitable counterstaining. As a control, CD352 (NTB-A) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD352 (NTB-A). Splenocytes from C57BL/6 were stained with CD352 (NTB-A) antibodies and with a suitable counterstaining. As a control, CD352 (NTB-A) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD352 (NTB-A). Splenocytes from C57BL/6 were stained with CD352 (NTB-A) antibodies and with a suitable counterstaining. As a control, CD352 (NTB-A) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD352 (NTB-A). Splenocytes from C57BL/6 were stained with CD352 (NTB-A) antibodies and with a suitable counterstaining. As a control, CD352 (NTB-A) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD352 (NTB-A). Splenocytes from C57BL/6 were stained with CD352 (NTB-A) antibodies and with a suitable counterstaining. As a control, CD352 (NTB-A) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD352 (NTB-A). Splenocytes from C57BL/6 were stained with CD352 (NTB-A) antibodies and with a suitable counterstaining. As a control, CD352 (NTB-A) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD352 (NTB-A). Splenocytes from C57BL/6 were stained with CD352 (NTB-A) antibodies and with a suitable counterstaining. As a control, CD352 (NTB-A) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD352 (NTB-A). Splenocytes from C57BL/6 were stained with CD352 (NTB-A) antibodies and with a suitable counterstaining. As a control, CD352 (NTB-A) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Fixation data

To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.

No fixation

Post fixation

Comparison of staining pattern on non-fixed and fixed cells using CD352 (NTB-A) (13G3). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using CD352 (NTB-A) (13G3). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD352 (NTB-A) Antibody, anti-mouse

Overview

Clone 13G3 recognizes the mouse CD352 antigen, a single-pass type I membrane protein, also known as SLAM family member 6 (SLAM6) or NK, T, and B cell antigen (NTB-A) belonging to the CD2 subfamily of the immunoglobulin superfamily. It is expressed on NK, T, and B cells. It undergoes tyrosine phosphorylation and associates with the Src homology 2 domain-containing protein (SH2D1A) as well as with SH2 domain-containing phosphatases. CD352 plays a role in NK cell cytotoxicity and T cell cytokine responses. It controls neutrophil functions and serves as a regulator of both innate and adaptive immune responses.

Alternative names

SLAM family receptor, SLAMF6, SLAM F6, LY108, Lymphocyte antigen 108, SLAM family member 6

Detailed product information

Technical specifications

Clone	13G3
Clonality	monoclonal
Isotype	mouse IgG2aκ
Isotype control	Isotype Control Antibody, mouse IgG2a
Host	mouse
Type of antibody	Primary antibodies
Species	mouse
Antigen	CD352 (NTB-A)
Alternative names of antigen	SLAM family receptor, SLAMF6, SLAM F6, LY108, Lymphocyte antigen 108, SLAM family member 6
Molecular mass of antigen [kDa]	36
Distribution of antigen	B cells, T cells, NK cells
Entrez Gene ID	30925
RRID	AB_2657680, AB_2657681, AB_2657682, AB_2657683, AB_2657684, AB_2657685, AB_2657688, AB_2657689, AB_2889561, AB_2889562

Resources for CD352 (NTB-A) Antibody, anti-mouse

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

References for CD352 (NTB-A) Antibody, anti-mouse

Publications

Howie, D. et al. (2005) Cutting edge: the SLAM family receptor Ly108 controls T cell and neutrophil functions. J Immunol 174(10): 5931-5935
Griewank, K. et al. (2007) Homotypic interactions mediated by Slamf1 and Slamf6 receptors control NKT cell lineage development. Immunity 27(5): 751-762
Peck, S. R. et al. (2000) Ly108: a new member of the mouse CD2 family of cell surface proteins. Immunogenetics 52(1-2): 63-72

Applications

Extended validation