Clone:
REA808
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
NK-p46, hNKp46, NCR1, LY94

Extended validation for CD335 (NKp46) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA808
9E2++
195314++
Cells were incubated with an excess of purified unconjugated CD335 (NKp46) (REA808) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD335 (NKp46). Human peripheral blood mononuclear cells (PBMCs) were stained with CD335 (NKp46) antibodies and with a suitable counterstaining. As a control, CD335 (NKp46) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD335 (NKp46). Human peripheral blood mononuclear cells (PBMCs) were stained with CD335 (NKp46) antibodies and with a suitable counterstaining. As a control, CD335 (NKp46) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD335 (NKp46). Human peripheral blood mononuclear cells (PBMCs) were stained with CD335 (NKp46) antibodies and with a suitable counterstaining. As a control, CD335 (NKp46) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD335 (NKp46). Human peripheral blood mononuclear cells (PBMCs) were stained with CD335 (NKp46) antibodies and with a suitable counterstaining. As a control, CD335 (NKp46) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD335 (NKp46). Human peripheral blood mononuclear cells (PBMCs) were stained with CD335 (NKp46) antibodies and with a suitable counterstaining. As a control, CD335 (NKp46) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD335 (NKp46). Human peripheral blood mononuclear cells (PBMCs) were stained with CD335 (NKp46) antibodies and with a suitable counterstaining. As a control, CD335 (NKp46) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD335 (NKp46). Human peripheral blood mononuclear cells (PBMCs) were stained with CD335 (NKp46) antibodies and with a suitable counterstaining. As a control, CD335 (NKp46) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD335 (NKp46) (REA808). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD335 (NKp46) (REA808). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD335 (NKp46) (REA808). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD335 (NKp46) Antibody, anti-human, REAfinity™

Overview

Clone REA808 recognizes the CD335 antigen, a type I membrane glycoprotein also known as NKp46. CD335 is a member of the natural cytotoxicity receptor (NCR) family which trigger cytotoxicity in natural killer (NK) cells and mediates cell activation. CD335 is directly involved in target cell recognition and lysis and is exclusively expressed on CD3
CD56
+
NK cells, suggesting it to be a universal marker for NK cells.
Additional information: Clone REA808 displays negligible binding to Fc receptors.

Alternative names

NK-p46, hNKp46, NCR1, LY94

Detailed product information

Technical specifications

CloneREA808
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD335 (NKp46)
Alternative names of antigenNK-p46, hNKp46, NCR1, LY94
Molecular mass of antigen [kDa]32
Distribution of antigenNK cells
Entrez Gene ID9437
RRIDAB_2657590, AB_2657591, AB_2657592, AB_2657593, AB_2657594, AB_2657595, AB_2657596, AB_2657597, AB_2734017, AB_2734018, AB_2657598, AB_2657599, AB_2811677

Resources for CD335 (NKp46) Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD335 (NKp46) Antibody, anti-human, REAfinity™

Publications

  1. Pessino, A. et al. (1998) Molecular cloning of NKp46: A novel member of the immunoglobulin superfamily involved in triggering of natural cytotoxicity. J. Exp. Med. 188: 953-960
  2. Sivori, S. et al. (2000) 2B4 functions as a co-receptor in human NK cell activation. Eur. J. Immunol. 30: 787-793
  3. Moretta, A. et al. (2001) Activating receptors and coreceptors involved in human natural killer cell-mediated cytolysis. Annu. Rev. Immunol. 19: 197-223
  4. Moretta, L. and Moretta, A. (2004) Unravelling natural killer function: Triggering and inhibitory human NK receptors. EMBO J. 23: 255-259
  5. Lai, C. B. et al. (2012) Role of runt-related transcription factor 3 (RUNX3) in transcription regulation of natural cytotoxicity receptor 1 (NCR1/NKp46), an activating natural killer (NK) cell receptor. J. Biol. Chem. 287(10): 7324-7334

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