Clone:
AC104.3E3
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1λ
Applications:
FC, MC
Alternative names:
Siglec-3, p67, My9

Extended validation for CD33 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with AC104.3E3
REA775++
HIM3-4-
Hu9a (Gemtuzumab Biosimilar)-
P67.6+
WM-53++
Cells were incubated with an excess of purified unconjugated CD33 (AC104.3E3) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD33. Human peripheral blood mononuclear cells (PBMCs) were stained with CD33 antibodies and with a suitable counterstaining. As a control, CD33 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD33. Human peripheral blood mononuclear cells (PBMCs) were stained with CD33 antibodies and with a suitable counterstaining. As a control, CD33 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD33. Human peripheral blood mononuclear cells (PBMCs) were stained with CD33 antibodies and with a suitable counterstaining. As a control, CD33 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD33. Human peripheral blood mononuclear cells (PBMCs) were stained with CD33 antibodies and with a suitable counterstaining. As a control, CD33 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD33. Human peripheral blood mononuclear cells (PBMCs) were stained with CD33 antibodies and with a suitable counterstaining. As a control, CD33 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD33. Human peripheral blood mononuclear cells (PBMCs) were stained with CD33 antibodies and with a suitable counterstaining. As a control, CD33 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD33. Human peripheral blood mononuclear cells (PBMCs) were stained with CD33 antibodies and with a suitable counterstaining. As a control, CD33 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD33. Human peripheral blood mononuclear cells (PBMCs) were stained with CD33 antibodies and with a suitable counterstaining. As a control, CD33 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD33 (AC104.3E3). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD33 (AC104.3E3). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD33 (AC104.3E3). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD33 Antibody, anti-human

Overview

Clone AC104.3E3 recognizes the human CD33 antigen, a 67 kDa glycoprotein belonging to the sialoadhesin superfamily. The CD33 antigen is highly expressed on human monocytes but weakly on granulocytes and some – but not all – myeloid dendritic cells. The CD33 antigen is also found on myeloid progenitor cells (CFU-GEMM, CFU GM, CFU-G, BFU-E) but is not expressed on lymphocytes, platelets, erythrocytes, or primitive hematopoietic stem cells.

Alternative names

Siglec-3, p67, My9

Detailed product information

Technical specifications

CloneAC104.3E3
Clonalitymonoclonal
Isotypemouse IgG1λ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
AntigenCD33
Alternative names of antigenSiglec-3, p67, My9
Molecular mass of antigen [kDa]38
Distribution of antigendendritic cells, granulocytes, Langerhans cells, macrophages, mast cells, monocytes, myeloid leukemia cells, NK cells, T cells, basophils, leukemia cells, granulocytes, dendritic cells, Langerhans cells, macrophages, mast cells, monocytes, myeloid leukemia cells, NK cells, T cells, basophils, leukemia cells
Entrez Gene ID945
RRIDAB_2726123, AB_2726401, AB_2726124, AB_2726397, AB_2726120, AB_2726403, AB_2726126, AB_2726402, AB_2726125, AB_2726398, AB_2726121, AB_2726399, AB_2726122, AB_2660358, AB_2726400

Resources for CD33 Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD33 Antibody, anti-human

Publications

  1. Bernstein, I. D. et al. (1987)
    Treatment of acute myeloid leukemia cells
    in vitro
    with a monoclonal antibody recognizing a myeloid differentiation antigen allows normal progenitor cells to be expressed.
    J. Clin. Invest. 79(4): 1153-1159

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