Clone:
REA605
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
JAM-1, JAM-A, PAM-1, F11 R

Extended validation for CD321 (JAM1) Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD321 (JAM1) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD321 (JAM1)-PE (REA605, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD321 (JAM1) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD321 (JAM1)-PE (REA605, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD321 (JAM1) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD321 (JAM1)-PE (REA605, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD321 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD321-PE, clone (REA605). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD321 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD321-PE, clone (REA605). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD321 (JAM1) Antibody, anti-human, REAfinity™

Overview

Clone REA605 recognizes the human CD321 antigen, a cell-surface adhesion molecule, also known as JAM1 or JAMA. CD321 is a type I transmembrane glycoprotein belonging to the immunoglobin superfamily. It has two extracellular Ig-like domains, a transmembrane region and a short cytosolic tail.
CD321 is expressed on a variety of cell types: erythrocytes, leukocytes, platelets, as well as endothelial and epithelial cells. CD321 has different functions. It is a regulator of tight junction assembly in epithelia and acts as a receptor for reovirus. Furthermore CD321 binds integrin LFA1 and is involved in leukocyte transmigration. In addition, CD321 is a platelet receptor.
Additional information: Clone REA605 displays negligible binding to Fc receptors.

Alternative names

JAM-1, JAM-A, PAM-1, F11 R

Detailed product information

Technical specifications

CloneREA605
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD321 (JAM1)
Alternative names of antigenJAM-1, JAM-A, PAM-1, F11 R
Molecular mass of antigen [kDa]30
Distribution of antigenendothelial cells, epithelial cells, platelets, red blood cells, leukocytes
Entrez Gene ID50848
RRIDAB_2904942, AB_2657454, AB_2657455, AB_2657456, AB_2657457, AB_2657458, AB_2657459, AB_2657460, AB_2657461, AB_2657462, AB_2657463, AB_2657464, AB_2657465, AB_2657466, AB_2657467, AB_2904943

Resources for CD321 (JAM1) Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD321 (JAM1) Antibody, anti-human, REAfinity™

Publications

  1. Fraemohs, L. et al. (2004) The functional interaction of the beta 2 integrin lymphocyte function-associated antigen-1 with junctional adhesion molecule-A is mediated by the I domain. J. Immunol. 173(10): 6259-6264
  2. Ozaki, H. et al. (1999) Cutting edge: combined treatment of TNF-alpha and IFN-gamma causes redistribution of junctional adhesion molecule in human endothelial cells. J. Immunol. 163(2): 553-557
  3. Sobocka, M. B. et al. (2000) Cloning of the human platelet F11 receptor: a cell adhesion molecule member of the immunoglobulin superfamily involved in platelet aggregation. Blood 95(8): 2600-2609

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