Clone:
2E1
Type of antibody:
Primary antibodies
Isotype:
mouse IgG2aκ, mouse IgG2a
Applications:
FC, MC, MICS, IF, IHC
Alternative names:
FCGR2A, CD32A, CDw32, FCG2, FCGR21, FcGR, IGFR2, FCGR2B, CD32B, FcγRII

Extended validation for CD32 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with 2E1
FUN-2+
FLIP8.26++
3D3+
6C4++
REA997++
Cells were incubated with an excess of purified unconjugated CD32 (2E1) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD32. Human peripheral blood mononuclear cells (PBMCs) were stained with CD32 antibodies and with a suitable counterstaining. As a control,CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD32. Human peripheral blood mononuclear cells (PBMCs) were stained with CD32 antibodies and with a suitable counterstaining. As a control,CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD32. Human peripheral blood mononuclear cells (PBMCs) were stained with CD32 antibodies and with a suitable counterstaining. As a control,CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD32. Human peripheral blood mononuclear cells (PBMCs) were stained with CD32 antibodies and with a suitable counterstaining. As a control,CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD32. Human peripheral blood mononuclear cells (PBMCs) were stained with CD32 antibodies and with a suitable counterstaining. As a control,CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD32. Human peripheral blood mononuclear cells (PBMCs) were stained with CD32 antibodies and with a suitable counterstaining. As a control,CD32 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD32 (2E1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD32 (2E1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD32 (2E1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD32 Antibody, anti-human

Overview

CD32, also known as FcγRII, is a 40 kDa low affinity receptor for the Fc fragment of aggregated IgG. Due to alternative splicing three different isoforms of CD32 are expressed on hematopoietic cells: FcRIIA, FcRIIB, and FcRIIC. The cytoplasmic domains of FcRIIA and FcRIIC contain an activatory ITAM motif whereas FcRIIB is an inhibitory receptor containing an ITIM motif. Different isoforms of CD32 are expressed on B cells, monocytes, dendritic cells, granulocytes, and platelets. The monoclonal antibody 2E1 recognizes all isoforms of CD32 without blocking the binding of immune complexes.

Alternative names

FCGR2A, CD32A, CDw32, FCG2, FCGR21, FcGR, IGFR2, FCGR2B, CD32B, FcγRII

Detailed product information

Technical specifications

Clone2E1
Clonalitymonoclonal
Isotypemouse IgG2aκ, mouse IgG2a
Isotype controlIsotype Control Antibody, mouse IgG2a
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, non-human primate, other
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
, baboon, horse
AntigenCD32
Alternative names of antigenFCGR2A, CD32A, CDw32, FCG2, FCGR21, FcGR, IGFR2, FCGR2B, CD32B, FcγRII
Molecular mass of antigen [kDa]31
Distribution of antigenB cells, dendritic cells, endothelial cells, granulocytes, Langerhans cells, macrophages, monocytes, NK cells, leukocytes, lymphocytes, basophils, eosinophils, neutrophils, plasma cells, placenta, B cells, dendritic cells, endothelial cells, granulocytes, Langerhans cells, leukocytes, lymphocytes, macrophages, monocytes, myeloid leukemia cells, NK cells, platelets, basophils, eosinophils, neutrophils, plasma cells, placenta
Entrez Gene ID2212
RRIDAB_2784136, AB_2657451, AB_2657443, AB_2657444, AB_2657446, AB_2657447, AB_2657450, AB_2784137

Resources for CD32 Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD32 Antibody, anti-human

Publications

  1. Båve, U. et al. (2003) Fc gamma RIIa is expressed on natural IFN-alpha-producing cells (plasmacytoid dendritic cells) and is required for the IFN-alpha production induced by apoptotic cells combined with lupus IgG. J. Immunol. 171(6): 3296-3302
  2. Van Den Herik-Oudijk, I. E. et al. (1994) Functional analysis of human Fc gamma RII (CD32) isoforms expressed in B lymphocytes. J. Immunol. 152(2): 574-585

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