Clone:
REA474
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
FCRL2, FcRH2, IFGP4, IRTA4, SPAP1, SPAP1A, SPAP1B, SPAP1C

Extended validation for CD307b (FcRL2) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA474
296902++
Cells were incubated with an excess of purified unconjugated CD307b (FcRL2) (REA474) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD307b (FcRL2) (REA474). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD307b (FcRL2) (REA474). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD307b (FcRL2) (REA474). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD307b (FcRL2) Antibody, anti-human, REAfinity™

Overview

Clone REA474 recognizes the human CD307b antigen, a single-pass type I membrane protein, which is also known as Fc receptor-like protein 2 (FcRL2) or immunoglobulin receptor translocation-associated protein 4 (IRTA4). CD307b is a protein with immunomodulatory potential that is preferentially expressed by memory B cells in humans. It has two consensus ITIMs in addition to a putative ITAM sequence in its cytoplasmic domain. Coligation of CD307b with the B cell receptor (BCR) leads to tyrosine phosphorylation of its ITIM motifs and subsequent Src homology region 2 domain-containing phosphatase-1 recruitment to facilitate inhibition of BCR signaling. Mutational analysis indicates that the tyrosine residues in both inhibitory motifs of CD307b are required for complete inhibition of BCR signaling, whereas tyrosines in the putative activation motif are dispensable for signal modulation. These findings suggest a negative immunomodulatory function for CD307b in the regulation of memory B cells.
Additional information: Clone REA474 displays negligible binding to Fc receptors.

Alternative names

FCRL2, FcRH2, IFGP4, IRTA4, SPAP1, SPAP1A, SPAP1B, SPAP1C

Detailed product information

Technical specifications

CloneREA474
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD307b (FcRL2)
Alternative names of antigenFCRL2, FcRH2, IFGP4, IRTA4, SPAP1, SPAP1A, SPAP1B, SPAP1C
Molecular mass of antigen [kDa]53
Distribution of antigenB cells
Entrez Gene ID79368
RRIDAB_2802024, AB_2657249, AB_2657250, AB_2657251, AB_2657252, AB_2802036

Resources for CD307b (FcRL2) Antibody, anti-human, REAfinity™

Certificates

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References for CD307b (FcRL2) Antibody, anti-human, REAfinity™

Publications

  1. Jackson, T. A. et al. (2010) FcR-like 2 inhibition of B cell receptor-mediated activation of B cells. J. Immunol. 185(12): 7405-7412
  2. Davis, R. S. et al. (2001) Identification of a family of Fc receptor homologs with preferential B cell expression. Proc. Natl. Acad. Sci. U.S.A. 98(17): 9772-9777
  3. Shabani, M. et al. (2014) Ligation of human Fc receptor like-2 by monoclonal antibodies down-regulates B-cell receptor-mediated signalling. Immunology 143(3): 341-353

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