Clone:
REA687
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
Clec10a, MGL, M-ASGP-BP-1

Extended validation for CD301 (CLEC10A) Antibody, anti-mouse, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD301 (CLEC10A) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD301 (CLEC10A)-PE (REA687, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD301 (CLEC10A) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD301 (CLEC10A)-PE (REA687, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD301 (CLEC10A) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD301 (CLEC10A)-PE (REA687, red) and counterstained with DRAQ5 (blue) as DNA stain.

Specifications for CD301 (CLEC10A) Antibody, anti-mouse, REAfinity™

Overview

Clone REA687 recognizes the mouse CD301 (CLEC10A) antigen, which is a 40 kDa type II transmembrane glycoprotein expressed by immature DCs and activated macrophages. Mouse CD301 has two homologue genes, CD301a (MGL1) and CD301b (MGL2). CD301 is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily which plays a role in cell adhesion, cell-cell signaling, glycoprotein turnover, inflammation, and immune response. CD301 binds in a calcium-dependent manner to terminal galactose and N-acetylgalactosamine units, linked to serine or threonine.
Additional information: Clone REA687 displays negligible binding to Fc receptors.

Alternative names

Clec10a, MGL, M-ASGP-BP-1

Detailed product information

Technical specifications

CloneREA687
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD301 (CLEC10A)
Alternative names of antigenClec10a, MGL, M-ASGP-BP-1
Molecular mass of antigen [kDa]35
Distribution of antigendendritic cells, macrophages
Entrez Gene ID17312
RRIDAB_2657164, AB_2657165, AB_2657166, AB_2657167, AB_2657168, AB_2657169, AB_2657170, AB_2657171, AB_2657172, AB_2657163

Resources for CD301 (CLEC10A) Antibody, anti-mouse, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD301 (CLEC10A) Antibody, anti-mouse, REAfinity™

Publications

  1. Denda-Nagai, K. et al. (2010) Distribution and function of macrophage galactose-type C-type lectin 2 (MGL2/CD301b): efficient uptake and presentation of glycosylated antigens by dendritic cells. J. Biol. Chem. 285(25): 19193-19204
  2. Tsuiji, M. et al. (2002) Molecular cloning and characterization of a novel mouse macrophage C-type lectin, mMGL2, which has a distinct carbohydrate specificity from mMGL1. J. Biol. Chem. 277(32): 28892-28901
  3. Singh, S. K. et al. (2009) Characterization of murine MGL1 and MGL2 C-type lectins: distinct glycan specificities and tumor binding properties. Mol. Immunol. 46(6): 1240-1249

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