Clone:
MIH18
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC, MICS, IF, IHC
Alternative names:
PDCD1LG2, B7-DC, Btdc, CD273, PD-L2, PDCD1L2, PDL2

Extended validation for CD273 (PD-L2) Antibody, anti-human

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD273 (PD-L2). Mature MoDc cells were stained with CD273 (PD-L2) antibodies and with a suitable counterstaining. As a control, CD273 (PD-L2) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD273 (PD-L2). Mature MoDc cells were stained with CD273 (PD-L2) antibodies and with a suitable counterstaining. As a control, CD273 (PD-L2) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD273 (PD-L2). Mature MoDc cells were stained with CD273 (PD-L2) antibodies and with a suitable counterstaining. As a control, CD273 (PD-L2) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD273 (PD-L2). Mature MoDc cells were stained with CD273 (PD-L2) antibodies and with a suitable counterstaining. As a control, CD273 (PD-L2) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD273 (PD-L2). Mature MoDc cells were stained with CD273 (PD-L2) antibodies and with a suitable counterstaining. As a control, CD273 (PD-L2) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD273 (PD-L2). Mature MoDc cells were stained with CD273 (PD-L2) antibodies and with a suitable counterstaining. As a control, CD273 (PD-L2) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD273 (PD-L2) (MIH18). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD273 (PD-L2) (MIH18). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD273 (PD-L2) (MIH18). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD273 (PD-L2) Antibody, anti-human

Overview

Clone MIH18 recognizes human CD273, also known as B7-DC and PD-L2. CD273 with an approximate molecular weight of 25 kDa is a type I transmembrane protein, belonging to the B7 family of protein ligands and to the Ig superfamily. CD273 is mainly expressed on dendritic cells (DCs) and activated macrophages. It has been shown to interact with programmed-death-receptor 1 (PD-1). Both a stimulatory and inhibitory role of CD273, in T cell activation has been proposed.

Alternative names

PDCD1LG2, B7-DC, Btdc, CD273, PD-L2, PDCD1L2, PDL2

Detailed product information

Technical specifications

CloneMIH18
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD273 (PD-L2)
Alternative names of antigenPDCD1LG2, B7-DC, Btdc, CD273, PD-L2, PDCD1L2, PDL2
Molecular mass of antigen [kDa]29
Distribution of antigendendritic cells, macrophages
Entrez Gene ID80380
RRIDAB_2656862, AB_2656863, AB_2656864, AB_2656865, AB_2656866, AB_2656867, AB_2656870, AB_2656871, AB_2656872, AB_2656873, AB_2752180, AB_2752199

Resources for CD273 (PD-L2) Antibody, anti-human

Certificates

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References for CD273 (PD-L2) Antibody, anti-human

Publications

  1. Tseng, S. Y. et al. (2001)
    B7-DC, a new dendritic cell molecule with potent costimulatory properties for T cells.
    J. Exp. Med. 193: 839-846
  2. Pfistershammer, K. et al. (2006) No evidence for dualism in function and receptors: PD-L2/B7-DC is an inhibitory regulator of human T cell activation. Eur. J. Immunol. 36(5): 1104-1113
  3. Greenwald, R. J. et al. (2005) The B7 family revisited. Annu. Rev. Immunol. 23: 515-548

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