Clone:
AD5-8E7
Type of antibody:
Primary antibodies
Isotype:
mouse IgG2a
Applications:
FC, MICS, IF, IHC, ICC, MC
Alternative names:
BDCA-1, CD1, R7, M241, T6, CD1a

Extended validation for CD1c (BDCA-1) Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with AD5-8E7
REA694++
L161-
F10/21A3-
Cells were incubated with an excess of purified unconjugated CD1c (BDCA-1) (AD5-8E7) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD1c (BDCA-1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD1c (BDCA-1) antibodies and with a suitable counterstaining. As a control, CD1c (BDCA-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD1c (BDCA-1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD1c (BDCA-1) antibodies and with a suitable counterstaining. As a control, CD1c (BDCA-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD1c (BDCA-1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD1c (BDCA-1) antibodies and with a suitable counterstaining. As a control, CD1c (BDCA-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD1c (BDCA-1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD1c (BDCA-1) antibodies and with a suitable counterstaining. As a control, CD1c (BDCA-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD1c (BDCA-1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD1c (BDCA-1) antibodies and with a suitable counterstaining. As a control, CD1c (BDCA-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD1c (BDCA-1). Human peripheral blood mononuclear cells (PBMCs) were stained with CD1c (BDCA-1) antibodies and with a suitable counterstaining. As a control, CD1c (BDCA-1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD1c (BDCA-1) (AD5-8E7). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD1c (BDCA-1) (AD5-8E7). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD1c (BDCA-1) (AD5-8E7). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD1c (BDCA-1) Antibody, anti-human

Overview

The clone AD5-8E7 recognizes human CD1c, also known as BDCA-1. CD1c is expressed on a major subpopulation of human myeloid dendritic cells (about 0.3% of blood leukocytes). Blood CD1c (BDCA-1)
+
myeloid dendritic cells are CD11c
high
, CD123
low
, CD4
+
, Lin
, CD45RO
+
, CD2
+
, CD16
, CD141 (BDCA-3)
low
, CD303 (BDCA-2)
, and CD304 (BDCA-4/Neuropilin-1)
. They express myeloid markers (CD13, CD33) as well as Fc receptors (CD32, CD64, FcεRI) and are of monocytoid appearance. A minor proportion of CD1c (BDCA-1)
+
myeloid dendritic cells expresses CD14 and CD11b. In blood, CD1c (BDCA-1) is also expressed on a subpopulation of CD19
+
small resting B lymphocytes. CD1c (BDCA-1) expression has also been detected on cortical thymocytes, on Langerhans cells, and on CD1a
+
dendritic cells generated
ex vivo
from monocytes or hematopoietic precursor cells. CD1c (BDCA-1)
+
myeloid dendritic cells have been designated as type-1 myeloid dendritic cells (MDC1s).
The CD1c antigen is a member of the CD1 family of proteins that are structurally related to MHC class I proteins and mediate the presentation of non-peptide antigens to T cells.

Alternative names

BDCA-1, CD1, R7, M241, T6, CD1a

Detailed product information

Technical specifications

CloneAD5-8E7
Clonalitymonoclonal
Isotypemouse IgG2a
Isotype controlIsotype Control Antibody, mouse IgG2a
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
AntigenCD1c (BDCA-1)
Alternative names of antigenBDCA-1, CD1, R7, M241, T6, CD1a
Molecular mass of antigen [kDa]36
Distribution of antigenB cells, dendritic cells, Langerhans cells, macrophages, monocytes, lymphocytes, T cells, myeloid leukemia cells, thymocytes, B cells, dendritic cells, Langerhans cells, lymphocytes, macrophages, monocytes, myeloid leukemia cells, T cells, thymocytes, B cells, dendritic cells, Langerhans cells, macrophages, monocytes, myeloid leukemia cells, T cells, lymphocytes, thymocytes
Entrez Gene ID911
RRIDAB_2726080, AB_2726360, AB_2726083, AB_2726358, AB_2726081, AB_2726355, AB_2726078, AB_2726359, AB_2726082, AB_2726356, AB_2726079, AB_2661165, AB_244309, AB_2726357

Resources for CD1c (BDCA-1) Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD1c (BDCA-1) Antibody, anti-human

Publications

  1. Carrion, J. et al. (2012) Microbial carriage state of peripheral blood dendritic cells (DCs) in chronic periodontitis influences DC differentiation, atherogenic potential. J. Immunol. 189(6): 3178-3187
  2. Contreras, V. et al. (2010) Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species. J. Immunol. 185(6): 3313-3325
  3. Mittag, D. et al. (2011) Human dendritic cell subsets from spleen and blood are similar in phenotype and function but modified by donor health status. J. Immunol. 186(11): 6207-6217
  4. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
  5. Bosco, M. C. et al. (2011)
    Hypoxia modulates the gene expression profile of immunoregulatory receptors in human mature dendritic cells: identification of TREM-1 as a novel hypoxic marker
    in vitro
    and
    in vivo
    .
    Blood 117(9): 2625-2639
  6. Hou, W. et al. (2012) Viral infection triggers rapid differentiation of human blood monocytes into dendritic cells. Blood 119(13): 3128-3131
  7. van de Ven, R. et al. (2011) Characterization of four conventional dendritic cell subsets in human skin-draining lymph nodes in relation to T-cell activation. Blood 118(9): 2502-2510
  8. MacDonald, K. P. A. et al. (2002) Characterization of human blood dendritic cell subsets. Blood 100: 4512-4520
  9. Brodie Miles, E. et al. (2013) Noncanonical dendritic cell differentiation and survival driven by a bacteremic pathogen. J. Leukoc. Biol. 94(2): 281-289
  10. Hémont, C. et al. (2013) Human blood mDC subsets exhibit distinct TLR repertoire and responsiveness. J. Leukoc. Biol. 93(4): 599-609
  11. Brigl, M. and Brenner, M. B. (2004) CD1: antigen presentation and T cell function. Annu. Rev. Immunol. 22: 817-890
  12. Grabbe, S. et al. (2000) Dendritic cells: multi-lineal and multi-functional. Immunol. Today 21: 431-433
  13. Peiser, M. et al. (2003) CD1a and CD1c cell sorting yields a homogeneous population of immature human Langerhans cells. J. Immunol. Methods 279: 41-53
  14. Krupna-Gaylord, M. A. et al. (2014) Induction of type I and type III interferons by Borrelia burgdorferi correlates with pathogenesis and requires linear plasmid 36. PLoS One 9(6): e100174
  15. Clark, F. J. et al. (2003) Origin and subset distribution of peripheral blood dendritic cells in patients with chronic graft-versus-host disease. Transplantation 75: 221-225
  16. Lebre, C. M. et al. (2003)
    BDCA-3
    hi
    dendritic cells: a novel subset with distinct phenotypical characteristics. Doctoral dissertation.
    University of Amsterdam, Netherlands
  17. Vanders, R. L. et al. (2013) Plasmacytoid dendritic cells and CD8 T cells from pregnant women show altered phenotype and function following H1N1/09 infection. J. Infect. Dis. 208(7): 1062-1070

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