Clone:
REA319
Type of antibody:
Recombinant antibodies, Primary antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, ICC
Alternative names:
PSGL-1, SELPLG

Extended validation for CD162 Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD162 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD162-PE (REA319, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD162 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD162-PE (REA319, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD162 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD162-PE (REA319, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD162 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD162-PE, clone (REA319). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD162 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD162-PE, clone (REA319). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD162 Antibody, anti-human, REAfinity™

Overview

Clone REA319 recognizes the human CD162 antigen, a single-pass type I membrane protein which is also known as P-selectin glycoprotein ligand 1 (PSGL-1). CD162 is expressed on neutrophils, monocytes, and most lymphocytes. It is the high affinity receptor for CD62P (P-selectin) on myeloid cells and stimulated T lymphocytes and mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. CD162 is also involved in homeostasis including stem cell homing to the thymus and mature T cell homing to secondary lymphoid organs. It has been found to bind the homeostatic chemokines CCL19 and CCL21 and to support the chemotactic response to these chemokines.
Additional information: Clone REA319 displays negligible binding to Fc receptors.

Alternative names

PSGL-1, SELPLG

Detailed product information

Technical specifications

CloneREA319
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyRecombinant antibodies, Primary antibodies
Specieshuman
AntigenCD162
Alternative names of antigenPSGL-1, SELPLG
Molecular mass of antigen [kDa]121
Distribution of antigenmonocytes, neutrophils
Entrez Gene ID9332
RRIDAB_2819490, AB_2655464, AB_2655465, AB_2655466, AB_2655467, AB_2655468, AB_2655469, AB_2655470, AB_2655471, AB_2655472, AB_2655473, AB_2819498

Resources for CD162 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD162 Antibody, anti-human, REAfinity™

Publications

  1. Frenette, P. S. et al. (2000)
    P-selectin glycoprotein ligand 1 (PSGL-1) is expressed on platelets and can mediate platelet-endothelial interactions
    in vivo
    .
    J. Exp. Med. 191(8): 1413-1422
  2. Sako, D. et al. (1993) Expression cloning of a functional glycoprotein ligand for P-selectin. Cell 75(6): 1179-1186
  3. Carlow, D. A. et al. (2009) PSGL-1 function in immunity and steady state homeostasis. Immunol. Rev. 230(1): 75-96
  4. Evani, S.J. et al. (2016)
    Biophysical regulation of
    Chlamydia pneumoniae
    -infected monocyte recruitment to atherosclerotic foci.
    Sci Rep 6

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