Anti-Prominin-1 MicroBeads, mouse

Name: Anti-Prominin-1 MicroBeads, mouse
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Anti-Prominin-1 MicroBeads, mouse

Anti-Prominin-1 MicroBeads have been developed for the isolation of mouse Prominin-1

⁺

neural progenitor cells.

Product data and images for Anti-Prominin-1 MicroBeads, mouse

Figures

Figure 1

Enrichment of Prominin-1

⁺

cells from mouse cerebellum (postnatal day 1) using Anti-Prominin-1 MicroBeads, a MiniMACS™ Separator, and MS Columns.

Mouse cerebellar cells before separation	Prominin-1 ^– cells
View details Anti-Prominin-1 MicroBeads, mouse Figure 1 Enrichment of Prominin-1 ⁺ cells from mouse cerebellum (postnatal day 1) using Anti-Prominin-1 MicroBeads, a MiniMACS™ Separator, and MS Columns.	View details Anti-Prominin-1 MicroBeads, mouse Figure 1 Enrichment of Prominin-1 ⁺ cells from mouse cerebellum (postnatal day 1) using Anti-Prominin-1 MicroBeads, a MiniMACS™ Separator, and MS Columns.
Prominin-1 ⁺ cells
View details Anti-Prominin-1 MicroBeads, mouse Figure 1 Enrichment of Prominin-1 ⁺ cells from mouse cerebellum (postnatal day 1) using Anti-Prominin-1 MicroBeads, a MiniMACS™ Separator, and MS Columns.

Specifications for Anti-Prominin-1 MicroBeads, mouse

Overview

Anti-Prominin-1 MicroBeads have been developed for the isolation of mouse Prominin-1

⁺

neural progenitor cells.

Detailed product information

Background information

Prominin-1 is a glycoprotein with close structural similarity to its human ortholog, CD133

¹

. Prominin-1 expression has been demonstrated on cells of different embryonic and adult tissues, including ES-derived committed and early progenitor cells

²

, the developing

³

and postnatal

⁴

central nervous system, kidney tubules

³

, and cells of the hematopoietic system

³

. In the developing nervous system prominin-1 is predominantly expressed on neuroepithelial cells and other epithelia. In the postnatal brain, prominin-1 expression has been found in areas of neurogenesis, such as the subventricular zone (SVZ). Prominin-1 is preferentially localized to plasma membrane protrusions of the apical membrane.

⁵

Columns

For positive selection: MS, LS, or autoMACS

^®

Columns. For depletion: LD or autoMACS Columns.

Resources for Anti-Prominin-1 MicroBeads, mouse

Documents and Protocols

Brochures/posters

Neuroscience - Inspiring technologies for creative scientists

Scientific posters

Semi-automated tissue dissociation and preserved epitope integrity optimize immunomagnetic sorting of neural cells

References for Anti-Prominin-1 MicroBeads, mouse

Publications

Weigmann, A. et al. (1997) Prominin, a novel microvilli-specific polytopic membrane proteine of the apical surface of epithelial cells, is targeted to plasmalemmal protrusions of non-epithelial cells. Proc. Natl. Acad. Sci. U.S.A. 94: 12425-12430
Kania, G. et al. (2005) Somatic stem cell marker prominin-1/CD133 is expressed in embryonic stem cell-derived progenitors. Stem Cells 23: 791-804
Corbeil, D. et al. (2001) Prominin: a story of cholesterol, plasma membrane protrusions and human pathology. Traffic 2: 82-91
Lee, A. et al. (2005) Isolation of neural stem cells from the postnatal cerebellum. Nat. Neurosci. 8: 723-729
Marzesco, A.-M. et al. (2005) Release of extracellular membrane particles carrying the stem cell marker prominin-1 (CD133) from neural progenitors and other epithelial cells. J. Cell. Sci. 118: 2849-2858

Data and images