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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
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The scientific community is working at full speed to fight severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2). Miltenyi Biotec is committed to assisting those researchers in getting to the bottom of SARS‑CoV‑2.
Our newly developed SARS-CoV-2 PepTivator Peptide Pools can be used to load antigen-presenting cells and to stimulate SARS-CoV-2–specific T cells. The T cells can then be detected and/or isolated for further research. We provide four different peptide pools, covering the sequence of three major structural SARS-CoV-2 proteins.
If you are interested in virus-specific PepTivator Products besides SARS-CoV-2, please have a look here.
PepTivator SARS-CoV-2 Prot_S:
PepTivator SARS-CoV-2 Prot_S, 6 nmol per peptide
PepTivator SARS-CoV-2 Prot_S, 60 nmol per peptide
PepTivator SARS-CoV-2 Prot_S1:
PepTivator SARS-CoV-2 Prot_S1, 6 nmol per peptide
PepTivator SARS-CoV-2 Prot_S1, 60 nmol per peptide
PepTivator SARS-CoV-2 Prot_S+:
PepTivator SARS-CoV-2 Prot_S+, 6 nmol per peptide
PepTivator SARS-CoV-2 Prot_S+, 60 nmol per peptide
PepTivator SARS-CoV-2 Prot_N:
PepTivator SARS-CoV-2 Prot_N, 6 nmol per peptide
PepTivator SARS-CoV-2 Prot_N, 60 nmol per peptide
PepTivator SARS-CoV-2 Prot_M:
Please note that data are derived from a single experiment and do not include multiple donors. Thus, the results should be considered as exemplary only and not as representative. Results may vary dependent on donor, time since infection, and used assays.
Selected references
The surface or spike glycoprotein (S) plays a critical role in coronavirus infection as it is crucial for recognition and fusion of the virus with the host cell membrane. Two different functional domains of the S protein are responsible for these individual processes: The S1 domain contains the surface binding site to the host cell receptor, the ACE2 receptor, whereas membrane fusion is mediated via the S2 domain. Furthermore, cleavage at the boundary of the S1 and S2 domains (aa residues 685 and 686) via cellular proteases primes the protein for entry into the host cell. Upon fusion of the viral envelope and the host cell membrane, the viral genome enters the host cell and viral replication starts.
The S protein, and especially the S1 domain, have been considered a potential key target for vaccines, therapeutics, and diagnostic approaches (PMID: 32106567, 32142651).
The PepTivator SARS-CoV-2 Prot_S covers selected immunodominant sequence domains of the spike protein (aa 304–338, 421–475, 492–519, 683–707, 741–770, 785–802, and 885–1273; GenBank MN908947.3, Protein QHD43416.1). SARS-CoV-2 Prot_S1 spans the complete N-terminal S1 domain of the spike protein (aa 1–692) and SARS-CoV-2 Prot_S+ a part of the C-terminal S2 domain (aa 689–895).
All three PepTivator Products can be combined to achieve a complete sequence coverage of the spike protein. Furthermore, the complete S2 domain (and parts of the S1 domain: aa 304–338, 421–475, and 492–519) is covered, when SARS-CoV-2 Prot_S and SARS-CoV-2 Prot_S+ are combined.
Prot_N stands for nucleocapsid phosphoprotein (N). The N protein has multiple functions in the coronavirus replication cycle. Its primary function is viral genome packaging. Furthermore, it is involved in virus transcription and interacts with the viral membrane during virion assembly.
The N protein has been suggested as a possible target for vaccine development (PMID: 32106567).
The PepTivator SARS‑CoV‑2 Prot_N covers the complete sequence of the SARS‑CoV‑2 N protein (GenBank MN908947.3, Protein QHD43423.2).
Prot_M stands for membrane glycoprotein (M). The M protein is the most abundant structural protein in the coronavirus envelope and thus defines viral morphogenesis. Additionally, the M protein is crucial for efficient virion assembly and involved in viral budding.
The M protein has been suggested as a possible target for vaccine development (PMID: 16423399).
The PepTivator SARS‑CoV‑2 Prot_M covers the complete sequence of the SARS‑CoV‑2 M protein (GenBank MN908947.3, Protein QHD43419.1).
All PepTivator SARS‑CoV‑2 products are available in two sizes (6 nmol and 60 nmol per peptide per vial) in research-grade quality.
PepTivator SARS-CoV-2 Prot_S:
PepTivator SARS-CoV-2 Prot_S, 6 nmol per peptide
PepTivator SARS-CoV-2 Prot_S, 60 nmol per peptide
PepTivator SARS-CoV-2 Prot_S1:
PepTivator SARS-CoV-2 Prot_S1, 6 nmol per peptide
PepTivator SARS-CoV-2 Prot_S1, 60 nmol per peptide
PepTivator SARS-CoV-2 Prot_S+:
PepTivator SARS-CoV-2 Prot_S+, 6 nmol per peptide
PepTivator SARS-CoV-2 Prot_S+, 60 nmol per peptide
PepTivator SARS-CoV-2 Prot_N:
PepTivator SARS-CoV-2 Prot_N, 6 nmol per peptide
PepTivator SARS-CoV-2 Prot_N, 60 nmol per peptide
PepTivator SARS-CoV-2 Prot_M:
PepTivator® Peptide Pools consist of short 15-mer peptides with 11-amino-acid overlaps, covering the amino acid sequence of a particular virus-, tumor-, or auto-antigen.
They bind to MHC class I as well as MHC class II complexes and thus are suitable for the antigen-specific stimulation of CD4+ and CD8+ T cells. Products containing 6 nmol per peptide are sufficient to stimulate up to 10⁸ cells and products containing 60 nmol per peptide are sufficient to stimulate up to 10⁹ cells.
Virus-specific PepTivator Peptide Pools can be used:
More references for PepTivator Peptide Pools can be found in our reference list available for download.
Reference list:
Find a complete workflow to activate, isolate, and detect virus-specific T cells here.
Protocols:
The following protocols are designed for the 96-well plate format (PepTivators HT). You can adapt the protocols to the standard vial sizes of PepTivator Peptide Pools by resuspending the product as described in the data sheet and applying 2 µL/well (of a 96 well plate):
If you are interested in the SARS‑CoV‑2 PepTivator Peptide Pools, please fill in the contact form below and we will come back to you:
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