Antigens for SARS-CoV-2 research

Antigens for SARS-CoV-2 research

The scientific community is relentlessly working to investigate and fight SARS-CoV-2. Miltenyi Biotec stands committed to assisting those researchers in getting on top of COVID-19. 

SARS-CoV-2 antigens and ACE2

SARS-CoV-2 antigens offer researchers the possibility to investigate virus-specific immune responses, including antigen-specific B cells and antibodies.  

All our antigens for SARS-CoV-2 research are available in different filling sizes and with or without biotinylation. Recombinantly expressed antigens are tagged with His and AviTag™. For more details, please vist the respective product pages linked in the table below.

Overview of our antigens for SARS-CoV-2 research

SARS-CoV-2 lineageProtein   Sequence partProduction systemProduct page
n/aACE2Full length (with CLD)HEK cellsRecombinant Human ACE2 (HEK)
Truncated (w/o CLD)Insect cellsRecombinant Human ACE2 (insect cells)
Wild-type variantSpike proteinSpike trimer (ectodomain)HEK cellsRecombinant SARS-CoV-2 Spike-Trimer (HEK)
Spike monomer (ectodomain)HEK cellsRecombinant SARS-CoV-2 Spike-Prot (HEK)
Furin cleavage site (and control)Chemical synthesisSARS-CoV-2 Furin Cleavage Site
SARS-CoV-2 Furin Cleavage Site Control
Receptor binding domain (RBD)HEK cellsRecombinant SARS-CoV-2 RBD (HEK)
Insect cellsRecombinant SARS-CoV-2 RBD (insect cells)
S1HEK cellsRecombinant SARS-CoV-2 Spike-S1 (HEK)
S2 Insect cellsRecombinant SARS-CoV-2 Spike-S2 (insect cells)
NucleoproteinFull lengthHEK cellsRecombinant SARS-CoV-2 Nucleoprotein
Envelope proteinC-terminusChemical synthesisSARS-CoV-2 Envelope C-term
N-terminus Chemical synthesisSARS-CoV-2 Envelope N-term
Helical segment 2 (H2)Chemical synthesisSARS-CoV-2 Envelope H2
Mutated variants B.1.1.7 (Alpha) 
spike protein
Spike monomer (ectodomain)HEK cellsRecombinant SARS-CoV-2 Spike-Prot B.1.1.7  (HEK)
Receptor binding domain (RBD)HEK cellsRecombinant SARS-CoV-2 RBD B.1.1.7 (HEK)
B.1.351 (Beta) 
spike protein
Spike monomer (ectodomain)HEK cellsRecombinant SARS-CoV-2 Spike-Prot B.1.351 (HEK) 
Receptor binding domain (RBD)HEK cellsRecombinant SARS-CoV-2 RBD B.1.351 (HEK)
B.1.617.2 (Delta) spike protein 
Spike monomer (ectodomain)HEK cellsRecombinant SARS-CoV-2 Spike-Prot B.1.617.2 (HEK)
Receptor binding domain (RBD)HEK cellsRecombinant SARS-CoV-2 RBD B.1.617.2 (HEK)
B.1.1.529/BA.1 (Omicron) spike protein Spike monomer (ectodomain)HEK cellsRecombinant SARS-CoV-2 Spike-Prot B.1.1.529/BA.1 (HEK)
Receptor binding domain (RBD)HEK cellsRecombinant SARS-CoV-2 RBD B.1.1.529/BA.1 (HEK)
Related products:

Find relevant products for analysis, enrichment, and culture of SARS-CoV-2–specific B cells

Are you interested in SARS-CoV-2–specific T cell research? Check out our SARS-CoV-2 PepTivator® Peptide Pools.

Applications of SARS-CoV-2 antigens

Overview of possible applications

  • Detection of patient-derived antibodies
  • Characterization of patient-specific immune responses
  • Detection and phenotyping of virus-specific B cells
  • Enrichment of virus-specific B cells
  • Analysis of virus binding to host cells
  • Protein detection assays

Detection of SARS-CoV-2–specific antibodies in serum samples

Detection of patient-derived antibodies against SARS-CoV-2 antigens via a bead-based immunoassay 

Biotinylated recombinant proteins coupled to streptavidin-coated PMMA beads can be applied to detect SARS-CoV-2–specific antibodies in plasmapheresis samples of COVID-19+ patients. The complete protocol can be found here.

Analysis of patient-derived SARS-CoV-2 antibodies
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Analysis of different plasma dilutions illustrates that our SARS-CoV-2 antigens enable a highly sensitive detection of patient-derived anti–SARS-CoV-2 antibodies. 

Quantification of captured SARS-CoV-2 antibodies at different plasma dilutions
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Quantification of captured SARS-CoV-2 antibodies at different plasma dilutions shows the high specificity of our SARS-CoV-2 antigens.

Download PDF

Knowing antibody titers is often the first step before analyzing the cellular immune response to an infection. To get an overview about our workflows for detection of humoral and cellular responses to SARS-CoV-2 download our scientific poster.

Detection of spike protein binding to ACE2

Cell-based assay to analyze the binding of spike protein to native ACE2

The combination of recombinant spike RBD, monomer or trimer, and ACE2-expressing cells can be used to mimic the binding of SARS-CoV-2 to its host cells in vitro. Such binding assays can be applied to study inhibitory/neutralizing effects of components or antibodies.

Analysis of spike protein binding to cell surface ACE2 
View details

Immunofluorescent staining visualizes spike proteins (RBD, monomer, trimer) bound to ACE2-expressing cells. Comparison of the percentage of stained cells at different protein concentrations shows a titratable, high-affinity binding of all three recombinant spike proteins to cell surface ACE2, with the trimer showing the highest affinity.  

This assay can be applied to analyze the potential of certain components to disrupt spike/ACE2 binding, which would result in a reduced number of stained cells. 

Furin cleavage screening assay 

Download PDF

Application note
Furin cleavage screening assay 

Furin cleavage of the S protein is considered a promising therapeutic target. Download this application note to see how our furin cleavage site peptides can be applied in a fluorogenic assay to screen for furin cleavage.

Contact us for more information

If you have further questions or are interested in our proteins for SARS-CoV-2 research, please specify your question or request in the contact form and we will come back to you:

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