PE buffer for dissociation of umbilical cord tissue

Prepare a solution with phosphate buffered saline (PBS), pH 7.2, and 2mM EDTA. Keep buffer cold (2–8 °C).
▲ Note: EDTA can be replaced by other supplements such as anticoagulant citrate dextrose formula-A (ACD-A) or citrate phosphate dextrose (CPD).

Dissociate human umbilical cord to a single-cell suspensions by combining mechanical dissociation with enzymatic degradation of the extracellular adhesion proteins that maintain the tissue structural integrity. Use the gentleMACS™ Octo Dissociator with Heaters in combination with the Umbilical Cord Dissociation Kit, human. Follow the protocol of the kit data sheet.
 

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Umbilical Cord Dissociation Kit, human

Expand umbilical cord-derived MSCs using the StemMACS™ MSC Expansion Media Kit XF, an optimized and standardized complete medium for the reproducible and reliable generation and expansion of MSCs from umbilical cord and other tissue sources. Follow the protocol of the kit data sheet.

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StemMACS MSC Expansion Media Kit XF, human

Growing interest in using MSCs for clinical research necessitates a fundamental understanding of mechanisms and processes underlying their differentiation into specific cell types, as well MSC phenotype validation of cultivated cells. The MSC Phenotyping Kit, human is based on ISCT (International Society for Cellular Therapy) markers for human MSCs, and helps easily perform standardized phenotyping of culture-expanded MSCs by flow cytometry. Follow the protocol of the kit data sheet. See "Functional analysis of expanded MSCs" to assess the differentiation potential of isolated MSCs.
 

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MSC Phenotyping Kit, human

Mesenchymal stem cell culturing protocols

To plan a dedicated antibody panel for your MSC research project, use our antibody panel builder.

Differentiation potential is a meaningful tool to qualify and define a MSC population. Grade of differentiation into adipocytes, chondrocytes, and osteoblasts is influenced by MSC origin, so for example, MSCs from bone marrow show greater potential to differentiate into osteoblasts than MSCs from umbilical cord. Increased age of donor and passaging of cultured MSCs also decrease differentiation potential.

StemMACS™ AdipoDiff, OsteoDiff, and ChondroDiff Media, human are optimized differentiation media for human MSCs isolated from various tissue sources. Each medium supports differentiation capacity analysis or quality control of expanded MSCs, as well as in vitro studies on MSC differentiation processes, including gene expression and protein profiling. Follow the protocol of the relevant kit data sheet.

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StemMACS AdipoDiff Media, human

StemMACS OsteoDiff Media, human

StemMACS ChondroDiff Media, human

In the last few years, scientists have directed their attention to the immunomodulatory potential of MSCs. MSCs derived from bone marrow have been observed to suppress proliferation of T cells.^1,2 This function of MSCs can be analyzed using the MSC Suppression Inspector, human, which contains T cell stimulation reagents optimized for an MSC suppression assay. Follow the protocol of the kit data sheet.

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MSC Suppression Inspector, human

1. Di Nicola, M. et al. (2002) Blood 99: 3838–3843.
2. Bartholomew, A. et al. (2002) Exp. Hematol. 30: 42–48.

The following is a list of relevant resources that might be of interest:

• Effective licensing of human mesenchymal stem cells
• Flyer: MSC Phenotyping Kit, human – Confirm compliance with ISCT criteria
  
• Flyer: StemMACS™ MSC Expansion Media Kit XF
  
• REAfinity™ Recombinant Antibodies – Flow cytometry is in their genes
• REAfinity Recombinant Antibodies – Frequently Asked Questions
  

Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.

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