T cell suppression assays

In this study by Janss et al., from ImmunXperts, a macrophage suppression assay was used to confirm the efficacy and potency of test compounds on the suppressive capacities of the macrophages on T cells. When comparing secretions of IFNγ and proliferation of CD4 T cells among untreated CD4 T cells alone, untreated CD4 T cells + M1 macrophage and CD4 T cells + M2 macrophages treated with different conditions highlights the suitability of our workflow solutions for a macrophage suppression assay for drug testing.

In this example study, the T reg suppression assay was used to assess the suppressive capacity of T regs on T cells upon treatment with three novel test compounds to assess their immunomodulatory potency. The resulting CFSE proliferating cell percentages allowed for a clear understanding on the action of the test compounds.