Application protocol

Engineering of CAR T cells
 for research use

Isolation of donor T cells, in vitro activation, transduction, expansion, phenotyping, and functional analysis

CAR T cells have to be developed, optimized and validated in a pre-clinical setting by small-scale benchtop experiments. This application protocol describes a complete workflow for the engineering of CAR T cells for research. It includes isolation of donor T cells, followed by activation, gene-transfer of the CAR construct, CAR T cell expansion as well as the phenotyping and analysis of the final CAR T cell product.

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Materials

The following is a listing of reagents, instruments, and consumables needed for each step of this protocol. These products are for research use only.
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Protocol


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This protocol includes detailed instructions for preparing necessary reagents and materials and then carrying out each step.