CD43 Glycoform antibodies, mouse

CD43 Glycoform antibodies, mouse

Clone: REA364 | Dilution: 1:10
Clone REA364 recognizes the activation-associated glycoform (130-kD glycoform) of the murine CD43 antigen, a cell-surface glycoprotein which is also known as sialophorin or leukosialin. CD43 is a heavily O-glycosylated protein which is expressed in a variety of hematopoietic lineage cells, including T lymphocytes. It has been reported to have two glycoforms, one with a molecular mass of 115 kD that is expressed on all T cells and one with a mass of 130 kD that is expressed only on activated T cells. Biochemical analysis showed that CD43 on resting T cells carries tetrasaccharides (115-kD glycoform), whereas activated T cells carry branched hexasaccharide core two O-glycans (130-kD glycoform). Expression of the 130-kD glycoform of CD43 on all T cells in transgenic mice shows a reduction in primary T-cell immune responses that is probably due to the antiadhesive function of this glycoform.
Additional information: Clone REA364 displays negligible binding to Fc receptors.

Alternative names

Spn, B-cell differentiation antigen LP-3, GALGP, Ly-48, Sialophorin, CD43

Technical specifications

  • Antigen: CD43 Glycoform
  • Clone: REA364
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: Spn, B-cell differentiation antigen LP-3, GALGP, Ly-48, Sialophorin, CD43
  • Entrez Gene ID: 20737
  • Molecular mass of antigen [kDa]: 38
  • Distribution of antigen: B cells, T cells, lymphocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, PE-Vio770, APC-Vio770, PerCP-Vio700
  • Selected references

    1. Carlow, D. A. et al. (1999) A novel CD8 T cell-restricted CD45RB epitope shared by CD43 is differentially affected by glycosylation J. Immunol. 163(3): 1441-1448
    2. van den Berg, T. K. et al. (2001) Cutting Edge: CD43 functions as a T cell counterreceptor for the macrophage adhesion receptor Sialoadhesin (Siglec-1). J. Immunol. 166: 3637-3640
    3. Alcaide, P. et al. (2007)
      The 130-kDa glycoform of CD43 functions as an E-selectin ligand for activated Tʜ1 cells
      in vitro
      and in delayed-type hypersensitivity reactions
      in vivo.
      J. Invest. Dermatol. 127(8): 1964-1972
Product options: 10
9 µg in 300 µL (1)
AUD 34.00
30 µg in 1 mL (1)
AUD 67.00
9 µg in 300 µL (1)
AUD 49.00
30 µg in 1 mL (1)
AUD 96.00
9 µg in 300 µL (1)
AUD 60.00
30 µg in 1 mL (1)
AUD 118.00
9 µg in 300 µL (1)
AUD 64.00
30 µg in 1 mL (1)
AUD 126.00
9 µg in 300 µL (1)
AUD 57.00
30 µg in 1 mL (1)
AUD 111.00

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