CD20 Cytoplasmic antibodies, human

CD20 Cytoplasmic antibodies, human

Clone: REA543 | Dilution: 1:11
Clone REA543 recognizes the cytoplasmic domain of the human CD20 antigen, a non-glycosylated transmembrane protein of 33–37 kDa that is expressed on B lineage cells from the pre–B cell stage to the B cell lymphoblast stage. The antigen is further expressed on most malignant B cells. CD20 is not found on early B cell progenitors or plasma cells. Oligomers of CD20 form a Ca
2+
channel and might function in the regulation of local responses during B cell activation.
In vitro
effects of CD20-specific antibodies on resting B cells indicate that CD20 is able to transduce an extracellular signal affecting the G0/G1 cell cycle transition. Studies have demonstrated that CD20-initiated intracellular signals involve tyrosine kinase activation and that CD20 is tightly associated with both serine and tyrosine kinases.
Additional information: Clone REA543 displays negligible binding to Fc receptors.

Technical specifications

  • Antigen: CD20 Cytoplasmic
  • Clone: REA543
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Entrez Gene ID: 931
  • Molecular mass of antigen [kDa]: 33
  • Distribution of antigen: B cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, PE-Vio770, APC-Vio770, PerCP-Vio700
  • Selected references

    1. Einfeld, D. A. et al. (1988) Molecular cloning of the human B cell CD20 receptor predicts a hydrophobic protein with multiple transmembrane domains. EMBO J. 7(3): 711-717
    2. Deans, J. P. et al. (1995) Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20. J. Biol. Chem. 270(38): 22632-22638
    3. Vaughan, A. T. et al. (2015) Activatory and inhibitory Fcγ receptors augment rituximab-mediated internalization of CD20 independent of signaling via the cytoplasmic domain. J. Biol. Chem. 290(9): 5424-5437
Product options: 10
for 30
tests
(1)
AUD 124.00
for 100
tests
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AUD 336.00
for 30
tests
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AUD 163.00
for 100
tests
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AUD 363.00
for 30
tests
(1)
AUD 163.00
for 100
tests
(1)
AUD 363.00
for 30
tests
(1)
AUD 146.00
for 100
tests
(1)
AUD 346.00
for 30
tests
(1)
AUD 146.00
for 100
tests
(1)
AUD 346.00

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