CD141 (BDCA-3) antibodies, human

CD141 (BDCA-3) antibodies, human

Clone: AD5-14H12 | Dilution: 1:50
Clone AD5-14H12 recognizes the human CD141 (BDCA-3) antigen which is expressed at high levels on a minor subpopulation of human myeloid dendritic cells (about 0.02% of blood leukocytes). CD141 (BDCA‑3)
high
blood dendritic cells are CD11c
dim
, CD123
, CD4
+
, Lin
, CD45RO
+
, CD2
, and CD16
. They express myeloid lineage markers, such as CD13 and CD33, and have a monocytoid morphology. Unlike CD1c (BDCA-1)
+
blood dendritic cells, CD141 (BDCA-3)
high
blood dendritic cells lack expression of CD2 and Fc receptors such as CD32, CD64, or FcεRI. CD141 (BDCA-3) is also present at very low levels on CD14
+
monocytes, granulocytes, CD303 (BDCA‑2)
+
CD304 (BDCA-4/Neuropilin-1)
+
plasmacytoid and CD1c (BDCA-1)
+
myeloid dendritic cells. CD141 (BDCA‑3)
high
CD1c (BDCA-1)
myeloid dendritic cells have been designated type-2 myeloid dendritic cells (MDC2s). CD141 is also known as thrombomodulin; thrombomodulin mediates co-agglutination by interaction with thrombin and protein C, though nothing is known about its function on MDC2s.

Alternative names

THBD, AHUS6, BDCA3, THPH12, THRM, TM

Technical specifications

  • Antigen: CD141 (BDCA-3)
  • Clone: AD5-14H12
  • Isotype: mouse IgG1κ
  • Isotype control: Mouse IgG1 – isotype control antibodies
  • Alternative names of antigen: THBD, AHUS6, BDCA3, THPH12, THRM, TM
  • Entrez Gene ID: 7056
  • Molecular mass of antigen [kDa]: 59
  • Distribution of antigen: endothelial cells, megakaryocytes, monocytes, myeloid cells, neutrophils, platelets, smooth muscle
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, VioBright FITC, PE, APC, VioBlue, PE-Vio770, APC-Vio770, Biotin, pure
  • Selected references

    1. Gosh, M. et al. (2012) CD13 regulates dendritic cell cross-presentation and T cell responses by inhibiting receptor-mediated antigen uptake. J. Immunol. 188(11): 5489-5499
    2. Mittag, D. et al. (2011) Human dendritic cell subsets from spleen and blood are similar in phenotype and function but modified by donor health status. J. Immunol. 186(11): 6207-6217
    3. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
    4. Chehimi, J. et al. (2002) Persistent decreases in blood plasmacytoid dendritic cell number and function despite effective highly active antiretroviral therapy and increased blood myeloid dendritic cells in HIV-infected individuals. J. Immunol. 168: 4769-4801
    5. Bosco, M. C. et al. (2011)
      Hypoxia modulates the gene expression profile of immunoregulatory receptors in human mature dendritic cells: identification of TREM-1 as a novel hypoxic marker
      in vitro
      and
      in vivo
      .
      Blood 117(9): 2625-2639
    6. Dutertre, C. A. et al. (2012)
      Pivotal role of M-DC8
      +
      monocytes from viremic HIV-infected patients in TNFα overproduction in response to microbial products.
      Blood 120(11): 2259-2268
    7. van de Ven, R. et al. (2011) Characterization of four conventional dendritic cell subsets in human skin-draining lymph nodes in relation to T-cell activation. Blood 118(9): 2502-2510
    8. MacDonald, K. P. A. et al. (2002) Characterization of human blood dendritic cell subsets. Blood 100: 4512-4520
    9. Chu, C. C. et al. (2012)
      Resident CD141 (BDCA3)
      +
      dendritic cells in human skin produce IL-10 and induce regulatory T cells that suppress skin inflammation.
      J. Exp. Med. 209(5): 935-945
    10. Jongbloed, S. L. et al. (2010)
      Human CD141
      +
      (BDCA-3)
      +
      dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens.
      J. Exp. Med. 207(6): 1247-1260
    11. Hémont, C. et al. (2013) Human blood mDC subsets exhibit distinct TLR repertoire and responsiveness. J. Leukoc. Biol. 93(4): 599-609
    12. Lebre, C. M. et al. (2003)
      BDCA-3
      hi
      dendritic cells: a novel subset with distinct phenotypical characteristics. Doctoral dissertation.
      University of Amsterdam, Netherlands
    13. Vanders, R. L. et al. (2013) Plasmacytoid dendritic cells and CD8 T cells from pregnant women show altered phenotype and function following H1N1/09 infection. J. Infect. Dis. 208(7): 1062-1070
    14. Jardine, L. et al. (2013) Rapid detection of dendritic cell and monocyte disorders using CD4 as a lineage marker of the human peripheral blood antigen-presenting cell compartment. Front Immunol 4: 495
    15. Oosterhoff, D. et al. (2012) Tumor-mediated inhibition of human dendritic cell differentiation and function is consistently counteracted by combined p38 MAPK and STAT3 inhibition. Oncoimmunology 1(5): 649-658
Product options: 17
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tests
(1)
AUD 358.00
for 30
tests
(1)
AUD 142.00
for 100
tests
(1)
AUD 344.00
for 30
tests
(1)
AUD 95.00
for 100
tests
(1)
AUD 278.00
100 µg in 1 mL
AUD 109.00

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