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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Human peripheral blood mononuclear cells (PBMCs) were stimulatedin vitro
for 5 hours with SEB in the presence of monensin and conjugated CD107a (LAMP-1) antibodies. Cells were harvested, fixed, permeabilized, and intracellularly stained with Anti-IFN-γ antibodies. Additionally, cell surface staining was performed with CD8 antibodies. Cells were analyzed by flow cytometry using the MACSQuant®
, IFN‑γ–secreting cells are shown.
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