Mouse IL-6

Mouse IL-6

IL-6 stands for interleukin 6. Mouse IL-6 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse IL-6 can be used for a variety of applications, including:
  • Induction of colony formation from hematopoietic progenitor cells in semi-solid medium.
  • Replacement of feeder cells in the preparation of murine hybridomas.
  • In vitro differentiation of TH 17 cells.
  • Cultivation of bone marrow cells for retroviral transduction.

Background information

Interleukin 6 (IL-6), originally identified as a B cell differentiation factor, is a multifunctional cytokine which regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions. It induces, for instance, the terminal maturation of activated B cells into antibody-secreting plasma cells and acts in synergy with IL-3 to support the proliferation of hematopoietic stem cells. IL-6 is produced by many cell types, such as monocytes, macrophages, fibroblasts, endothelial cells, and T cells. Disturbed IL-6 production has been associated with pathological processes, including inflammatory autoimmune diseases and cancer.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of 7TD1 cells (NIBSC 93/730)
  • premium grade: ≥ 4×
    10
    8
    U/mg
  • research grade: ≥ 2×
    10
    8
    U/mg
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 6
E. coli
10 µg
-
E. coli
25 µg
-
E. coli
10 µg
-
E. coli
25 µg
-
E. coli
100 µg
-
E. coli
1000 µg
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