Mouse IL-2 IS

Mouse IL-2 IS

IL-2 IS stands for interleukin 2 “improved sequence” and is a variant of IL-2 with the serine substituted for the native cysteine at amino acid position 160.
Mouse IL-2 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse IL-2 IS can be used for a variety of applications, including:
  • In vitro activation and propagation of mouse T cells, e.g., using the T Cell Activation/Expansion Kit, mouse.
  • In vitro activation and expansion of NK cells.
  • Generation of lymphokine-activated killer (LAK) cells.

Background information

Interleukin 2 (IL-2), a potent lymphoid cell growth factor, is a typical four α-helix bundle cytokine. IL-2 is produced by activated T cells, especially the CD4
+
T helper cell population. It plays an important role in both the activation and maintenance of immune responses and in lymphocyte development. IL-2 promotes proliferation and differentiation of T cells, NK cells, and B cells and is involved in the elimination of self-reactive T cells. IL-2 signals through a receptor complex consisting of IL-2 receptor α-chain (CD25), β- and common γ-chain. The later two are also used for IL-15 signaling.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of mouse CTLL-2 cells
  • premium grade: ≥ 2×
    10
    7
    U/mg
    (typical activity: ≥ 2×
    10
    7
    U/mg
    )
  • research grade: ≥ 8×
    10
    6
    U/mg
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 6
E. coli
10 µg
AUD 97.00
E. coli
25 µg
AUD 190.00
E. coli
10 µg
AUD 124.00
E. coli
25 µg
AUD 237.00
E. coli
100 µg
AUD 525.00
E. coli
1000 µg
AUD 2,126.00