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Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with SEB for six hours. After two hours, brefeldin A was added for stimulated and unstimulated cells. The cells were fixed, permeabilized, and intracellularly stained with Anti-IFN-γ antibodies as well as with CD4 antibodies and CD69 antibodies. Cells were then analyzed by flow cytometry using the MACSQuant® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. CD4+ lymphocytes were pre-gated for the analysis. Cell debris were excluded from the analysis based on scatter signals.
Clone | 45-15 |
---|---|
Clonality | monoclonal |
Isotype | mouse IgG1κ, mouse IgG1 |
Isotype control | Isotype Control Antibody, mouse IgG1 |
Host | mouse |
Type of antibody | Primary antibodies, Functional-grade antibodies |
Species | human, non-human primate |
Cross-reactivity | rhesus monkey ( Macaca mulatta) cynomolgus monkey ( Macaca fascicularis) |
Antigen | IFN-γ |
Alternative names of antigen | IFNg, IFG, IFI |
Molecular mass of antigen [kDa] | 16 |
Entrez Gene ID | 3458 |
RRID | AB_2733589, AB_2733718, AB_2733719, AB_2751182, AB_2751116, AB_2751183, AB_2751117, AB_2733079, AB_2733080, AB_10830868, AB_2733588 |
Clone | 45-15 |
---|---|
Clonality | monoclonal |
Isotype | mouse IgG1κ, mouse IgG1 |
Isotype control | Isotype Control Antibody, mouse IgG1 |
Host | mouse |
Type of antibody | Primary antibodies, Functional-grade antibodies |
Species | human, non-human primate |
Cross-reactivity | rhesus monkey ( Macaca mulatta) cynomolgus monkey ( Macaca fascicularis) |
Antigen | IFN-γ |
Alternative names of antigen | IFNg, IFG, IFI |
Molecular mass of antigen [kDa] | 16 |
Entrez Gene ID | 3458 |
RRID | AB_2733589, AB_2733718, AB_2733719, AB_2751182, AB_2751116, AB_2751183, AB_2751117, AB_2733079, AB_2733080, AB_10830868, AB_2733588 |
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