Human IL-15

Name: Human IL-15
Availability: InStock

Human IL-15

Recombinant human IL-15 (interleukin 15) promotes proliferation of activated T, NK, and B cells . The regulatory cytokine IL-15 has important diverse roles in the immune system. It is structurally related to IL-2 and they share many functional characteristics. The recombinant protein is optimized for use in cell culture, differentiation studies, and functional assays.

Product data and images for Human IL-15

Figures

Figure 1

**Human IL-15 activity assay.**
The biological activity of Human IL-15 is determined by proliferation assay using CTLL2 cells.

Figure 1

Human IL-15 activity assay.

The biological activity of Human IL-15 is determined by proliferation assay using CTLL2 cells.

Figure 2

**SDS-PAGE of Human IL-15, premium grade**
under reduced (R) and non-reduced (NR) conditions.

Figure 2

SDS-PAGE of Human IL-15, premium grade

under reduced (R) and non-reduced (NR) conditions.

Figure 3

Mass spectrometry analysis (ESI-MS) of Human IL-15, premium grade. The peak corresponds to the calculated molecular mass of 12770 Da.

Figure 3

Mass spectrometry analysis (ESI-MS) of Human IL-15, premium grade. The peak corresponds to the calculated molecular mass of 12770 Da.

Specifications for Human IL-15

Overview

Recombinant human IL-15 (interleukin 15) promotes proliferation of activated T, NK, and B cells . The regulatory cytokine IL-15 has important diverse roles in the immune system. It is structurally related to IL-2 and they share many functional characteristics. The recombinant protein is optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-15 can be used for a variety of applications, including:

Activation and expansion of NK and NKT cells.
In vitro differentiation of NK cells, e.g., from purified CD34⁺ cells.
In vitro T cell expansion, e.g., of naive CD8⁺ T cells, and T cell activation, e.g., of CTLs.

Detailed product information

Background information

IL-15 is a member of the four α-helix bundle cytokine family. It is produced by different cell types, including epithelial cells, monocytes, muscle and placenta cells. IL-15 is a potent lymphoid cell growth factor. It stimulates the proliferation of activated T cells and promotes the generation of cytotoxic T lymphocytes (CTLs). IL-15 also induces the generation, proliferation, and activation of NK cells as well as B cell growth and immunoglobulin production. In addition, IL-15 is important for the maintenance of CD8

⁺

memory T cells. For binding and signaling IL-15 uses the unique IL-15 receptor α-chain, but shares the β- and γ-chain of the IL-2 receptor.

Biological activity

Proliferation of CTLL-2 cells (NIBSC 95/554)
research grade: ≥ 2×
10
⁶
U/mg
premium grade: ≥ 5×
10
⁶
U/mg

(Typical specific activity: ≥ 1.1×
10
⁷
U/mg
)
We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity, exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade

cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human IL-15

Documents and Protocols

Background information

Premium-grade cytokine benefits

App notes/customer reports

How to switch to optimal activity-based cytokine dosing

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

References for Human IL-15

Publications

Hoerster, K. et al. (2021) HLA Class I Knockout Converts Allogeneic Primary NK Cells Into Suitable Effectors for "Off-the-Shelf" Immunotherapy. Front Immunol 11: 586168
Nicolas-Boluda, A. et al. (2021) Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment. Elife 10: e58688
Alzubi, J. et al. (2020) Automated generation of gene-edited CAR T cells at clinical scale. Mol Ther Methods Clin Dev. 20: 379-388
Alzubi, J. et al. (2020) PSMA-Directed CAR T Cells Combined with Low-Dose Docetaxel Treatment Induce Tumor Regression in a Prostate Cancer Xenograft Model. Mol. Ther. Oncolytics 18: 226-235
Arcangeli, S. et al. (2020) Next-Generation Manufacturing Protocols Enriching T SCM CAR T Cells Can Overcome Disease-Specific T Cell Defects in Cancer Patients. Front Immunol 11: 1217
Galletti, G. et al. (2020)
Two subsets of stem-like CD8
⁺
memory T cell progenitors with distinct fate commitments in humans.
Nat Immunol 21(12): 1552-1562
Olden, B. R. et al. (2019) Cell-Templated Silica Microparticles with Supported Lipid Bilayers as Artificial Antigen-Presenting Cells for T Cell Activation. Adv Healthc Mater. 8(2): e1801188
Formenti, S. C. et al. (2018) Radiotherapy Induces Responses of Lung Cancer to CTLA-4 Blockade. Nat Med 24(12): 1845-1851
Rotolo, A. et al. (2018) Enhanced Anti-lymphoma Activity of CAR19-iNKT Cells Underpinned by Dual CD19 and CD1d Targeting. Cancer Cell 34(4): 596-610
Fuchs, S. et al. (2014)
Patients with T
^+/low
NK
⁺
IL-2 receptor γ chain deficiency have differentially-impaired cytokine signaling resulting in severe combined immunodeficiency.
Eur. J. Immunol. 44(10): 3129-3140
Alvarez-Breckenridge, C. A. et al. (2012) The histone deacetylase inhibitor valproic acid lessens NK cell action against oncolytic virus-infected glioblastoma cells by inhibition of STAT5/T-BET signaling and generation of gamma interferon. J. Virol. 86(8): 4566-4577
Gordy, L. E. et al. (2011) IL-15 regulates homeostasis and terminal maturation of NKT cells. J Immunol 187(12): 6335-6345
Dietz, L. et al. (2010) Tracking human contact allergens: from mass spectrometric identification of peptide-bound reactive small chemicals to chemical-specific naive human T-cell priming. Toxicol. Sci. 117(2): 336-347
Juelke, K. et al. (2010)
CD62L expression identifies a unique subset of polyfunctional CD56
^dim
NK cells.
Blood 116(8): 1299-1307
Soman, G. et al. (2009) MTS dye based colorimetric CTLL-2 cell proliferation assay for product release and stability monitoring of interleukin-15: assay qualification, standardization and statistical analysis. J. Immunol. Methods 348: 83-94

Data and images