CD3 MicroBeads, human

CD3 MicroBeads were developed for positive selection or depletion of CD3
+
T cells from peripheral blood, bronchial lavage, cell culture, or various tissues such as lymphoid, nasal, and tumor tissue.

Data and images for CD3 MicroBeads, human

Figures

Figure 1

View details
Separation of CD3
+
cells from PBMCs using CD3 MicroBeads and a MiniMACS™ Separator with an MS Column.

Figure 1

Separation of CD3
+
cells from PBMCs using CD3 MicroBeads and a MiniMACS™ Separator with an MS Column.

Specifications for CD3 MicroBeads, human

Overview

CD3 MicroBeads were developed for positive selection or depletion of CD3
+
T cells from peripheral blood, bronchial lavage, cell culture, or various tissues such as lymphoid, nasal, and tumor tissue.

Detailed product information

Background information

CD3 is expressed on all T cells and CD56
+
NKT cells, and is associated with the T cell receptor. 70–80% of human peripheral blood lymphocytes and 65–85% of thymocytes are CD3
+
. The epitope recognized by CD3 MicroBeads is located on the CD3ε chain.

Downstream applications

T cells isolated by MACS
®
Technology have been used for various studies, e.g., on T cell cytotoxicity, T cell activation
1
, HIV infectivity
2
, signal transduction, and surface marker expression.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

Resources for CD3 MicroBeads, human

Documents and Protocols

Brochures/posters

T cell research - The complete product portfolio

Brochures/posters

MACS® Cell Pre-Enrichment for flow sorting and analysis

Reviews for CD3 MicroBeads, human

Human CD Separation Beads Review

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CD3 MicroBeads, human (130-050-101)

We are interested in enhancing the capacity of T cells to mount responses against HIV infection. We perform in vitro killing assays where CTLs activity is tested in presence of HIV-1 infected cells. For different experiments we purify CD3 cells to separate the T cell population and then proceed with other separation steps and different assays.

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References for CD3 MicroBeads, human

Publications

  1. Pitti, R. M. et al. (1998) Genomic amplification of a decoy receptor for Fas ligand in lung and colon cancer. Nature 396: 699-703
  2. Heath, S. L. et al. (1995) Follicular dendritic cells and human immunodeficiency virus infectivity. Nature 377: 740-744
  3. Lorenzen, D. R. et al. (1999) Immunoglobulin A1 protease, an exoenzyme of pathogenic Neisseriae, is a potent inducer of proinflammatory cytokines. J. Exp. Med. 190: 1049-1058
  4. Heidenreich, F. and Jovin, T. (1996) Synthesis of anti-acetylcholine receptor antibodies by CD5- B cells from peripheral blood of myasthenia gravis patients. J. Neurol. 243: 57-62
  5. Klein, U. et al. (1994) Variable region gene analysis of B cell subsets derived from a 4-year-old child: somatically mutated memory B cells accumulate in the peripheral blood already at young age. J. Exp. Med. 180: 1383-1393
  6. Nicoll, G. et al. (2003) Ganglioside GD3 expression on target cells can modulate NK cell cytotoxicity via siglec-7-dependent and -independent mechanisms. Eur. J. Immunol. 33: 1642-1648

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