recombinant human IgG1
Alternative names:
CD19 antigen, CD19 protein

Specifications for CD19 CAR Detection Reagent, human


The CD19 CAR Detection Reagent, anti-human, Biotin, REAfinity™ has been developed for the detection of transduced T cells that are engineered to express CD19-specific chimeric antigen receptors (CAR) on the cell surface, which recognize human CD19 antigen.
The CD19 CAR Detection Reagent is an antigen based detection reagent conjugated to biotin. It contains a recombinantly expressed fusion protein consisting of the human CD19 extracellular domains and a specifically mutated human IgG1 Fc region.
The engineered CD19 CAR T cells can be detected via the recognition of the CD19 protein, and identified by flow cytometry via anti-biotin fluorochromes. The mutated human IgG1 Fc region of the CD19 CAR Detection Reagent abolishes their binding to Fcγ receptors. This allows for background-free analysis and eliminates the need for additional blocking steps, such as using a FcR blocking reagent.

Alternative names

CD19 antigen, CD19 protein

Detailed product information

Technical specifications

Isotyperecombinant human IgG1
AntigenCD19 CAR
Alternative names of antigenCD19 antigen, CD19 protein
Distribution of antigenCAR T cells, CAR NK cells

References for CD19 CAR Detection Reagent, human


  1. Mosti, L. et al. (2021) Targeted multi-epitope switching enables straightforward positive/negative selection of CAR T cells. Gene Ther. 28(9): 602-612
  2. Ayuk, F. et al. (2020) Excellent proliferation and persistence of allogeneic donor-derived 41-BB based CAR-T cells despite immunosuppression with cyclosporine A. Haematologica 105(6): 322-324
  3. Badbaran, A. et al. (2020) Accurate In-Vivo Quantification of CD19 CAR-T Cells after Treatment with Axicabtagene Ciloleucel (Axi-Cel) and Tisagenlecleucel (Tisa-Cel) Using Digital PCR. Cancers (Basel) 12(7): 1970
  4. Alzubi, J. et al. (2021) Automated generation of gene-edited CAR T cells at clinical scale. Mol Ther Methods Clin Dev. 20: 379-388
  5. Cordes, N. et al. (2021) Anti-CD19 CARs displayed at the surface of lentiviral vector particles promote transduction of target-expressing cells. Mol Ther Methods Clin Dev. 21: 42-53
  6. Lo Presti, V. et al. (2021)
    Efficient lentiviral transduction method to gene modify cord blood CD8
    T cells for cancer therapy applications.
    Mol Ther Methods Clin Dev. 21: 357-368
  7. Wullenkord, R. et al. (2021) Sequential therapy with inotuzumab ozogamicin, CD19 CAR T cells, and blinatumomab in an elderly patient with relapsed acute lymphoblastic leukemia. Ann Hematol. 100(2): 587-589
  8. Demaret, J. et al. (2021) Monitoring CAR T-cells using flow cytometry. Cytometry B Clin Cytom. 100: 218-224

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