When starting from solid tissue, the Mitochondria Extraction Kit – Tissue enables the automatic and standardized generation of tissue homogenates containing intact mitochondria.
The kit works in combination with gentleMACS™ Dissociators and integrates gentle tissue homogenization and cell lysis in a single step. The resulting tissue homogenates provide the ideal basis for subsequent magnetic isolation of intact and functional mitochondria with the Mitochondria Isolation Kits.
Accelerate your mitochondria isolation process with our easy to use Mitochondria Isolation Kits for human and mouse samples. Our kits use Anti-TOM22 MicroBeads to enable the isolation of pure and functional mitochondria with high yields in less than two hours. Mitochondria isolated with our kits, are suitable for downstream molecular and functional assays.
High-purity isolated mitochondria
Compared to differential centrifugation (DC) and ultracentrifugation (UC), mitochondria isolated with our Mitochondria Isolation Kits show much less contamination from other cell organelles.
Enrichment of functional mitochondria
Our workflow is gentle to cells and cellular organelles. Isolated mitochondria maintain their integrity and give reliable and reproducible data in functional assays.
Free Application note – Synaptic mitochondria isolation
Novel use of our Mitochondria Isolation Kits to isolate, purify, and separate non-synaptic and synaptic mitochondria from brain.
Scientific references
- Hubbard, W.B. et al. (2019) Fractionated mitochondrial magnetic separation for isolation of synaptic mitochondria from brain tissue. Sci Rep. 9(1).
- Franko, A. et al. (2013) Efficient isolation of pure and functional mitochondria from mouse tissues using automated tissue disruption and enrichment with anti-TOM22 magnetic beads. PloS One, 8(12), e82392.
- Hornig-Do, H.T. et al. (2009) Isolation of functional pure mitochondria by superparamagnetic microbeads. Anal Biochem. 389(1): 1–5.
- Xin, H. et al. (2019) Quantum biological tunnel junction for electron transfer imaging in live cells. Nat Commun. 10(1).
- Adav, S. S. et al. (2019) Quantitative profiling brain proteomes revealed mitochondrial dysfunction in Alzheimer’s disease. Mol Brain. 12(1).
