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Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 +CD25 high CD127 dim/–cells. |
A: | B: |
An appropriate threshold was defined based on CD45-VioBlue versus side scatter signals, for the exclusion of debris and erythrocytes from the data. | Lymphocytes were gated for their expected morphology using a forward scatter versus side scatter "lymphocyte gate" (P1) |
Treg Detection Kit (CD4/CD25/CD127), humanFigure 1Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 +CD25 high CD127 dim/–cells. | Treg Detection Kit (CD4/CD25/CD127), humanFigure 1Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 +CD25 high CD127 dim/–cells. |
C: | D: |
Dead cells were excluded by a CD127-PE versus propidium iodide dot plot (P2). | CD4 + lymphocytes were gated in P3. |
Treg Detection Kit (CD4/CD25/CD127), humanFigure 1Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 +CD25 high CD127 dim/–cells. | Treg Detection Kit (CD4/CD25/CD127), humanFigure 1Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 +CD25 high CD127 dim/–cells. |
E: | |
CD25 highCD127 dim/– Treg cells were gated by a CD127-PE versus CD25-APC dot plot. | |
Treg Detection Kit (CD4/CD25/CD127), humanFigure 1Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 +CD25 high CD127 dim/–cells. |
Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 +CD25 high CD127 dim/–cells. |
A: | B: |
An appropriate threshold was defined based on CD45-VioBlue versus side scatter signals, for the exclusion of debris and erythrocytes from the data. | Lymphocytes were gated for their expected morphology using a forward scatter versus side scatter "lymphocyte gate" (P1) |
Treg Detection Kit (CD4/CD25/CD127), humanFigure 1Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 +CD25 high CD127 dim/–cells. | Treg Detection Kit (CD4/CD25/CD127), humanFigure 1Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 +CD25 high CD127 dim/–cells. |
C: | D: |
Dead cells were excluded by a CD127-PE versus propidium iodide dot plot (P2). | CD4 + lymphocytes were gated in P3. |
Treg Detection Kit (CD4/CD25/CD127), humanFigure 1Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 +CD25 high CD127 dim/–cells. | Treg Detection Kit (CD4/CD25/CD127), humanFigure 1Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 +CD25 high CD127 dim/–cells. |
E: | |
CD25 highCD127 dim/– Treg cells were gated by a CD127-PE versus CD25-APC dot plot. | |
Treg Detection Kit (CD4/CD25/CD127), humanFigure 1Example of immunofluorescent staining with the Treg Detection Kit (CD4/CD25/CD127) using whole blood and the lyse/no wash protocol with CD45 trigger. A–E depict the gating strategy for the flow cytometric analysis of CD4 +CD25 high CD127 dim/–cells. |
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