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Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with 1 µg/mL soluble CD3 antibodies and 5 µg/mL soluble CD28 antibodies for two days. After incubation cells were washed and incubated with 50 ng/mL IL-4 and 20 ng/mL IL-2 for 4 days. Cells were then restimulated with PMA and ionomycin for 2 hours and brefeldin A was added for additionally four hours. Cells were then fixed and intracellularly stained with Anti-TNF-β antibodies as well as with CD3 antibodies. Flow cytometry was performed using the MACSQuant® Analyzer. Cell debris were excluded from the analysis based on scatter signals.
Clone | REA1169 |
---|---|
Clonality | monoclonal |
Isotype | recombinant human IgG1 |
Isotype control | REA Control Antibody (I), human IgG1 |
Host | human cell line |
Type of antibody | Primary antibodies, Recombinant antibodies |
Species | human |
Antigen | TNF-β |
Alternative names of antigen | TNF-beta, TNF-b, LT-alpha, LT-α |
Molecular mass of antigen [kDa] | 19 |
Distribution of antigen | T cells, B cells |
Entrez Gene ID | 4049 |
RRID | AB_2801793, AB_2801796, AB_2801794, AB_2801795 |
Clone | REA1169 |
---|---|
Clonality | monoclonal |
Isotype | recombinant human IgG1 |
Isotype control | REA Control Antibody (I), human IgG1 |
Host | human cell line |
Type of antibody | Primary antibodies, Recombinant antibodies |
Species | human |
Antigen | TNF-β |
Alternative names of antigen | TNF-beta, TNF-b, LT-alpha, LT-α |
Molecular mass of antigen [kDa] | 19 |
Distribution of antigen | T cells, B cells |
Entrez Gene ID | 4049 |
RRID | AB_2801793, AB_2801796, AB_2801794, AB_2801795 |
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