MZ and FO B Cell Isolation Kit, mouse

MZ and FO B Cell Isolation Kit, mouse

The Marginal Zone and Follicular B Cell Isolation Kit has been developed for the isolation of marginal zone (MZ) B cells and follicular (FO) B cells from mouse spleen.

Data and images
Specifications

Product data and images for MZ and FO B Cell Isolation Kit, mouse

Figures

Figure 1

MZ and FO B cells were isolated from mouse spleen cell suspension by using the Marginal Zone and Follicular B Cell Isolation Kit, mouse. The cells were fluorescently stained with CD45R (B220)- VioBlue (# 130-094-287), CD21/CD35-PE-Vio770 (# 130-097-216), CD23-PE (# 130-097-819), and CD93 (AA4.1)-APC and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before isolation

Figure 1

MZ and FO B cells were isolated from mouse spleen cell suspension by using the Marginal Zone and Follicular B Cell Isolation Kit, mouse. The cells were fluorescently stained with CD45R (B220)- VioBlue (# 130-094-287), CD21/CD35-PE-Vio770 (# 130-097-216), CD23-PE (# 130-097-819), and CD93 (AA4.1)-APC and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

MZ and FO B cells were isolated from mouse spleen cell suspension by using the Marginal Zone and Follicular B Cell Isolation Kit, mouse. The cells were fluorescently stained with CD45R (B220)- VioBlue (# 130-094-287), CD21/CD35-PE-Vio770 (# 130-097-216), CD23-PE (# 130-097-819), and CD93 (AA4.1)-APC and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Flow-through fraction: unlabeled enriched MZ B cells

Figure 1

MZ and FO B cells were isolated from mouse spleen cell suspension by using the Marginal Zone and Follicular B Cell Isolation Kit, mouse. The cells were fluorescently stained with CD45R (B220)- VioBlue (# 130-094-287), CD21/CD35-PE-Vio770 (# 130-097-216), CD23-PE (# 130-097-819), and CD93 (AA4.1)-APC and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

MZ and FO B cells were isolated from mouse spleen cell suspension by using the Marginal Zone and Follicular B Cell Isolation Kit, mouse. The cells were fluorescently stained with CD45R (B220)- VioBlue (# 130-094-287), CD21/CD35-PE-Vio770 (# 130-097-216), CD23-PE (# 130-097-819), and CD93 (AA4.1)-APC and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Eluted fraction: enriched FO B cells

Figure 1

MZ and FO B cells were isolated from mouse spleen cell suspension by using the Marginal Zone and Follicular B Cell Isolation Kit, mouse. The cells were fluorescently stained with CD45R (B220)- VioBlue (# 130-094-287), CD21/CD35-PE-Vio770 (# 130-097-216), CD23-PE (# 130-097-819), and CD93 (AA4.1)-APC and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

MZ and FO B cells were isolated from mouse spleen cell suspension by using the Marginal Zone and Follicular B Cell Isolation Kit, mouse. The cells were fluorescently stained with CD45R (B220)- VioBlue (# 130-094-287), CD21/CD35-PE-Vio770 (# 130-097-216), CD23-PE (# 130-097-819), and CD93 (AA4.1)-APC and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for MZ and FO B Cell Isolation Kit, mouse

Overview

The Marginal Zone and Follicular B Cell Isolation Kit has been developed for the isolation of marginal zone (MZ) B cells and follicular (FO) B cells from mouse spleen.

Detailed product information

Background information

The isolation of CD21

^hi

CD23

^lo/–

marginal zone (MZ) and CD21

^int

CD23

^hi

follicular (FO) B cells is performed in a two-step procedure. First, the non-B cells, the B-1 cells, and the transitional B cells are indirectly magnetically labeled with a cocktail of biotin-conjugated antibodies, as primary labeling reagent, and Anti-Biotin MicroBeads, as secondary labeling reagent. In between the two labeling steps no washing steps are required. The labeled cells are subsequently depleted by separation over a MACS

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Column, which is placed in the magnetic field of a MACS Separator. The unlabeled fraction contains the pre-enriched MZ and FO B cells. In order to further separate these two subsets, the FO B cells are directly labeled with CD23 MicroBeads in a second step and isolated by separation over a MACS Column, which is placed in the magnetic field of a MACS Separator. The flow-through contains the enriched MZ B cells. After removing the column from the magnetic field, the magnetically retained FO B cells can be eluted as the positively selected cell fraction.

Columns

LS or autoMACS

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Columns.

Data and images