CD62L antibodies, human

Clone: REA615 | Dilution: 1:50
Clone REA615 recognizes the human CD62L antigen, a 74 kDa single-pass type I membrane protein, which is also known as L-selectin, LECAM-1, or LAM-1. CD62L is a member of the selectin family of cell surface molecules and binds a series of glycoproteins including CD34, GlyCAM-1, and MAdCAM-1. Most hematopoietic cells express CD62L, including peripheral blood B cells, T cells, monocytes, granulocytes, thymocytes, and some myeloid cells from bone marrow. Expression on resting naive, central memory, and some effector memory T cells regulates their migration via endothelial venules to peripheral lymph nodes and Peyer’s patches. The CD62L antigen also contributes to the recruitment of leukocytes from the blood to areas of inflammation. Disruption of CD62L expression has detrimental effects on T cell migration and immune responses. Antigenic activation in the lymph node leads first to rapid CD62L shedding by protease cleavage and then to transcriptional suppression.
Always use fresh material for immunofluorescent staining of CD62L
cells. For optimal results, the cells should not be older than 8–12 hours. Keep cells continuously cold. CD62L-expression may be rapidly lost due to shedding.
Additional information: Clone REA615 displays negligible binding to Fc receptors.

Alternative names

L-selectin, LAM-1, LECAM-1, Leu-8, TQ1, gp90-MEL

Technical specifications

  • Antigen: CD62L
  • Clone: REA615
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: L-selectin, LAM-1, LECAM-1, Leu-8, TQ1, gp90-MEL
  • Entrez Gene ID: 6402
  • Molecular mass of antigen [kDa]: 38
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    cynomolgus monkey (
    Macaca fascicularis
    , baboon
  • Distribution of antigen: B cells, bone marrow, eosinophils, granulocytes, Langerhans cells, lymphocytes, monocytes, neutrophils, NK cells, red blood cells, spleen, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, Biotin
  • Selected references

    1. Ivetic, A. (2013) Signals regulating L-selectin-dependent leucocyte adhesion and transmigration. Int. J. Biochem. Cell Biol. 45(3): 550-555
    2. McEver, R. P. (2015) Selectins: initiators of leucocyte adhesion and signalling at the vascular wall. Cardiovasc. Res. 107(3): 331-339
    3. Telen, M. J. (2014) Cellular adhesion and the endothelium: E-selectin, L-selectin, and pan-selectin inhibitors. Hematol. Oncol. Clin. North Am. 28(2): 341-354
Product options: 4

CD62L-PE, human

for 30
EUR  90,00

CD62L-PE, human

for 100
EUR  220,00
for 30
EUR  75,00
for 100
EUR  185,00

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