Anti-LGR5 antibodies, mouse

Anti-LGR5 antibodies, mouse

Clone: DA04-10E8.9 | Dilution: 1:10
Clone DA04-10E8.9 recognizes the mouse LGR5 antigen, also known as leucine-rich-repeat-containing G-protein–coupled receptor 5 or Gpr49 but not with its close homologues LGR4 or LGR6. LGR5 associates with Wnt-receptors and act as R-spondin receptor thereby playing a central role in the modulation of Wnt/β-catenin signaling in normal and neoplastic stem cells. Initially described as a highly specific marker for stem cells in the small intestine, colon, hair follicle, stomach, and during kidney development, LGR5 positive cells were also shown to be crucial during the development and progression of cancer. It was shown that LGR5 positive crypt stem cells are the cells-of-origin in intestinal cancer and that CSCs in human colorectal cancer can be identified and isolated based on LGR5 expression.

Alternative names

Fex, GPR49

Technical specifications

  • Antigen: LGR5
  • Clone: DA04-10E8.9
  • Isotype: rat IgG2b
  • Isotype control: Rat IgG2b – isotype control antibodies
  • Alternative names of antigen: Fex, GPR49
  • Entrez Gene ID: 14160
  • Molecular mass of antigen [kDa]: 97
  • Distribution of antigen: cancer stem cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: Biotin
  • Selected references

    1. Hermey, G. et al. (1999) Identification of a novel seven-transmembrane receptor with homology to glycoprotein receptors and its expression in the adult and developing mouse. Biochem. Biophys. Res. Commun. 254(1): 273-279
    2. Morita, H. et al. (2004) Neonatal lethality of LGR5 null mice is associated with ankyloglossia and gastrointestinal distension. Mol. Cell. Biol. 24(22): 9736-9743
    3. Barker, N. et al. (2007) Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature 449(7165): 1003-1007
    4. Jaks, V. et al. (2008) Lgr5 marks cycling, yet long-lived, hair follicle stem cells. Nat. Genet. 40: 1291-1299
Product options: 2
9 µg in 300 µL (1)
EUR  21,00
30 µg in 1 mL (1)
EUR  45,00

Related products

1 products available