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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left image) or stimulated with 20 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 2 hours, followed by an incubation with 1 µg/mL brefeldin A for 4 hours. Cells were fixed, permeabilized, and directly stained with Anti-IL-3 antibodies as well as with CD154 antibodies. Flow cytometry was performed using the MACSQuant®
cells were pregated for the analysis. Cell debris were excluded from the analysis based on scatter signals.