Anti-AKT pS473 antibodies, human and mouse

Clone: REA359 | Dilution: 1:11
Clone REA359 recognizes the human and mouse Akt antigen phosphorylated at serine 473 (pS473). Akt is a family of serine/threonine kinases which is also known as protein kinase B (PKB) or related to the A and C kinases (RAC-PK). Akt intricately regulates many cellular functions such as cell growth and proliferation, cell survival, apoptosis, energy metabolism, and resistance to anticancer therapeutics. It consists of three isoforms, Akt1, Akt2, and Akt3 (or PKBα, β, and γ). Although these three isoforms are encoded by separate genes, they share a common NH2-terminal pleckstrin homology (PH) domain, a catalytic domain in the middle, and a COOH terminus. The identity of the overall amino acid sequence of the three isoforms is very high (about 80%); however, the COOH terminus and the PH-linker region are more diverse. All three isoforms contain similar phosphorylation/activation sites: threonine 308 (Akt1), 309 (Akt2), and 305 (Akt3) and serine 473 (Akt1), 474 (Akt2), and 472 (Akt3). Phosphorylated or activated Akt regulates many of its cellular functions through the phosphorylation of a series of downstream players.
Additional information: Clone REA359 displays negligible binding to Fc receptors.

Alternative names


Technical specifications

  • Antigen: AKT pS473
  • Clone: REA359
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: Akt1, CWS6, PKB, PKB-ALPHA, RAC, RAC-ALPHA, PKBγ, PKBβ, STK-2
  • Entrez Gene ID: 11651, 207
  • Molecular mass of antigen [kDa]: 56
  • Distribution of antigen: other
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE-Vio770, Vio515
  • Selected references

    1. Calamito, M. et al. (2010) Akt1 and Akt2 promote peripheral B-cell maturation and survival. Blood 115: 4043-4050
    2. Arranz, A. et al. (2012) Akt1 and Akt2 protein kinases differentially contribute to macrophage polarization. Proc. Natl. Acad. Sci. U.S.A. 109(24): 9517-9522
    3. Santi, S. A. et al. (2010) The Akt isoforms are present at distinct sub cellular locations. Am. J. Physiol., Cell Physiol. 298(3): 580-591
Product options: 4
for 30
EUR  100,00
for 100
EUR  240,00
for 30
EUR  95,00
for 100
EUR  230,00

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