StraightFrom® Whole Blood MicroBeads enable magnetic cell separation directly from whole blood. Leukocyte subsets such as CD4+, CD8+, CD14+, CD19+, or CD56+ cells can be purified using either cell separation instruments or manual separators. Pre-enrichment of white blood cells, e.g. by density gradient centrifugation, is not required. The purified cells are well-suited for further flow cytometric analyses, molecular biology applications, and functional studies.
Separation of a whole blood sample using the StraightFrom® Whole Blood CD3 MicroBeads, human and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns without washing step after labeling. Cells were fluorescently stained with CD3-PE, CD14‑APC, as well as CD45-VioBlue®and analyzed by flow cytometry using the MACSQuant® Analyzer.
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