Cell isolation directly from Leukopak®

  • Isolation of highly pure cells straight from Leukopak® in 30 minutes
  • No density gradient centrifugation, no erythrocyte lysis
  • Ready-to-use kits and easy protocols for automated cell isolation

StraightFrom® Leukopak® MicroBeads allow for the magnetic cell separation of large leukocyte numbers directly from Leukopak® (StemExpress, LCC). Leukocyte subsets such as CD4+, CD8+, CD14+, or CD19+ cells can be purified using either semi-or fully automated using MultiMACS™ Instruments. Pre-enrichment of white blood cells, e.g. by density gradient centrifugation, is not required. The purified cells are well-suited for further flow cytometric analyses, molecular biology applications, and functional studies.

Leukopak® as starting material

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During leukapheresis, white blood cells are being separated from the donor's blood and collected as highly concentrated leukocytes in Leukopaks® . Therefore, they provide the ideal source for the isolation of larges numbers of  leukocytes from PBMCs.

Automated cell isolation with the MultiMACS Cell24 Separator Plus

Semi-automated cell isolation directly from Leukopak® with the MultiMACS™ Cell24 Separator Plus.
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The MultiMACS Cell24 Separator Plus is perfectly suited for the semi-automated isolation of large cell numbers from Leukopaks® using StraightFrom Leukopak® MicroBeads.

The isolation procedure results in cell populations with high purities and recovery rates. With the Multi-24 Column Block (included in the kit), the instrument allows for time saving processing of large sample volumes, while providing reliability reproducibility in day-to-day cell separation. 

For large-scale cell separations, the MultiMACS X is the instrument of choice.

Data

Separation of CD14+ cells using StraightFrom Leukopak® CD14 MicroBeads

CD14+ cells can be isolated straight from a Leukopak® sample using the StraightFrom Leukopak® CD14 MicroBead Kit, human and the MultiMACS™ Cell24 Separator Plus.

Before separation
After separation

Separation of a Leukopak® sample using the StraightFrom Leukopak® CD14 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Multi-24 Column Block. Cells were fluorescently stained with CD14-APC, CD15-PE-Vio® 770, as well as CD45-VioBlue® and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Videos

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Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products using the MultiMACS™ Cell24 Separator Plus.

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Webinar

Immune cell isolation without density gradient centrifugation

Learn from this webinar how to isolate immune cells straight from blood or blood products without density gradient centrifugation.

 

 

Resources

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Brochure

StraightFrom MicroBeads - Cell isolation directly from blood products

Download the brochure to get all the details on our StraightFrom MicroBead Technology.

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Scientific poster

Isolation of functional cells straight from buffy coat, LRSC, and Leukopak®

Maike Schlemminger, Silke Weber-Lohmann, Martin Kolnik*, Zhong Yu, Susanne Höher-Peters, and Gregor Winkels

Miltenyi Biotec, Bergisch Gladbach, Germany

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